脂多糖体外刺激对小鼠B10细胞的影响

目的:探索脂多糖(LPS)体外刺激对小鼠B10细胞的影响。方法:在LPS刺激下,体外培养小鼠脾脏和腹腔细胞5h或48h,流式细胞仪分析CD19+IL-10+B细胞比例。结果:PIM刺激5h,小鼠脾脏和腹腔CD19+IL-10+B细胞比例分别升高至1.22%和14.82%(P<0.01),在PIM刺激的基础上加入LPS,小鼠脾脏和腹腔中CD19+IL-10+B细胞比例分别升高至平均2.35%和26.58%(P<0.01)。LPS刺激48h可进一步升高CD19+IL-10+B细胞比例,脾脏和腹腔分别为6.42%和38.38%,明显高于PIM48h刺激组(P<0.01)和L+PIM5h刺激组(P<0.05),但LPS刺激48h不改变CD5、CD1d的表达水平。结论:体外刺激5h时,L+PIM刺激可促进小鼠B10细胞活化;刺激48h时,LPS可进一步促进B10细胞IL-10的表达,这一作用可能是通过诱导B10前体细胞成熟实现的。

The effect of LPS stimulation in vitro on B10 cells of mice

Abstract] Objective： To explore the influence of LPS stimulation in vitro on the B10 cells of mice. Methods： Spleen and Peritoneal cavity cells of mice were cultured in vitro with LPS stimulator for 5h or 48h, then analyzed the percentage of the CD19＋IL-10＋B cells with flow cytometry. Results： The percentages of CD19＋IL-10＋B cells in spleen and peritoneal cavity of mice were raised to 1.22% and 14.82% with PIM stimulation for 5h（P〈0.01）. The mean percentages of CD19~IL-10＋B cells in spleen and peritoneal cavity of mice after L＋PIM stimulation for 5h were respectively 2.35% and 26.58%（P〈0.01）. And the frequencies of CD19＋IL-10＋B cells after L＋PIM stimulation for 48 h were respectively 6.42% and 38.38% and higher significantly than stimulation with PIM for 48 h（P〈0.01） and L＋PIM stimulation for 5h（P〈0.05）. However, L＋PIM stimulation for 48h don＇t change the expression of CD5 and CDld of spleen B cells. Conclusion： L＋PIM stimulation for 5h can pormote the activation of 10 cells of mice. LPS stimulation for 48h can further raise the expression of IL-10 in B10 cells of mice and this effect may be caused by inducing the maturation of B10pro in CD5＋CD1dhhi B cell subset. Key words] B10 cells; Interleukino-10; LPS; Culture in vitro; Flow cytometry