肺癌组织p16和Rb基因mRNA表达的双重原位杂交观察

目的：研究Ｐ１６和Ｒｂ基因在ｍＲＮＡ表达及其与肺癌的关系。方法：制备ｐ１６和Ｒｂ基因ｃＤＮＡ探针，采用双重原位杂交的方法，地８９例肺癌和正常肺组织进行了Ｐ１６ 一Ｒｂ基因ｍＲＮＡ表达的定位研究。结果：小细胞肺癌ｐ１６ｍＲＮＡ阳性表达率高达８２．４％，而非小细胞肺癌阳性率为４５．８％，两者差异有显著性。非小细胞肺癌ＲｂｍＲＮＡ阳性表达率为８１．９％，而小细胞肺癌一率仅５．９％，两者差异有高度显著性，

Observation of p16 and Rb gene mRNA expressions in lung cancers by in situ hybridization

Purpose To study p16 and Rb gene mRNA expressions and their relationships to lung cancer. Methods Preparation of p16 and Rb cDNA probes and in situ hybridization were applicated to locate p16 and Rb mRNA expressions in 89 cases of lung cancers and lung tissues. Results The positive expression rate of p16 mRNA in small cell lung cancers was 82 4%(14/17), but 45 8%(33/72) in non small cell lung cancers. The positive expression rate of Rb mRNA in non small cell lung cancers was 81 9%(59/72), but only 5 9%(1/17) in small cell lung cancers. The expressions of these two genes at protein and mRNA levels fundamentally corresponded with each other. Conclusion The loss of p16 mRNA expression mainly takes place in non small cell lung cancers, and loss of Rb mRNA expressions mainly takes place in small cell lung cancers. Maybe there is a mechanism at gene translation level which leads to the inactivation of p16 or Rb gene protein.