食管癌及癌旁组织中EGR-1、c-fos、cyclin D1的表达

目的：研究ＥＧＲ－１、ｃ－ｆｏｓ、ｃｙｃｌｉｎＤ１３种基因ｍＲＮＡ及其相应蛋白在人食管癌癌变过程中的表达水平及其相关性，并探讨其与食管癌的发生、发展及生物学行为的关系。方法：原位杂交法检测４７例食管鳞状细胞癌及其癌旁、上切缘中３种基因的ｍＲＮＡ、免疫组化ＳＡＢＣ法检测蛋白表达水平。结果：３种基因原位杂交、免疫 化阳性表达产物均分别定位于胞浆及胞核中，ＥＧＲ－１ｍＲＮＡ、ｃｆｏｓｍＲＮＡ、ｃｙｃｌｉ

Study on expressions of EGR-1, c-fos and cyclin D1 in human esophageal carcinoma

Purpose To study the expressions and correlations of mRNAs and proteins of EGR 1, c fos and cyclin D1 in human esophageal canceration course,and to probe the relationship between the expression of them in esophageal cancinoma(EC) tissues and the occurrence, development and biology behavior of EC. Methods In situ hybridization and immunohistochemistry were used respectively to detect mRNAs and proteins in 47 cases of esophageal squamous cell carcinoma and their correspondent para cancerous mucosae, distal cuts. Results In situ hybridization of the three genes and immunohistochemistry showed that positive products were all located in the cytoplasms and nuclei. Over expressions of EGR 1 mRNA, c fos mRNA, cyclin D1 mRNA and their proteins were found in dysplasias and squamous carcinomas. The expressions of EGR 1 and c fos were high in dysplasia mucosa, cyclin D1 was low, when it be came to cancer, EGR 1 expression decreased, c fos continued, cyclin D1 increased. The expressions between mRNA and protein of either of three genes were of consistence in their correspondent highest expression lesions. Conclusion EGR 1, c fos, cyclin D1 mRNAs and proteins express differently in various kinds of lesions in esophageal canceration course. The transition and development of esophageal canceration course would be determined by the interaction and supression among oncogenes and tumor suppressor genes.