肿节风防治化疗后血小板减少症的研究

目的:探讨肿节风对大剂量化疗后小鼠血小板减少的防治作用.方法:本研究以Babl/c纯系清洁级小鼠为实验对象,通过大剂量5-氟尿嘧啶腹腔注射复制血小板减少的动物模型,用肿节风稀释液从实验开始连续进行灌胃干预,分别在预处理前、化疗前、化疗后第2、第4及第7天测定小鼠血常规;并在化疗后第7天处死小鼠,行骨髓病理活检,观察骨髓造血情况.结果:经过肿节风预处理后,化疗前单药干预组及联合干预组小鼠外周血小板数量显著高于同期对照组(P＜0.05),化疗后两个实验组小鼠的血小板数量与对照组相比无显著下降(P＜0.05),而两实验组间无显著性差异(P＞0.05).骨髓病理检查结果提示,两实验组小鼠骨髓增生活跃,骨髓腔内可见巨核细胞,对照组小鼠骨髓增生低下,未发现巨核细胞.结论:肿节风具有对抗大剂量5-FU所造成的血小板减少的作用,从而预防并治疗化疗后血小板减少症的发生.

The Study on Effect of Sarcandra glabra on Prevention and Treatment of Thrombocytopenia by Chemotherapy

OBJECTIVE: To investigate the effect of Sarcandra glabra Thunb on prevention and treatment of thrombocytopenia after chemotherapy with a large dosage. METHODS: The Babl/c mice model of thrombocytopenia was made by intraperitoneal injection of 5-FU at a large dosage. All mice were poured Sarcandra glabra Thunb dilution to the mice's stomach once daily from the beginning of the experiment. Then the effect was analyzed by measuring peripheral blood cell counts of the mice on the 2nd day, the 4th day and the 7th day after chemotherapy. On the end of 7th day, all mice were killed, and then bone marrow biopsy was employed to investigate the hemapoietic condition. RESULTS: Before the chemotherapy, the platelet counts of mice treated by Sarcandra glabra Thunb were higher than that in the control group (P < 0.05). After chemotherapy with 5-FU, the platelet counts were not significantly decreased in two all experimental groups compared with negative control group (P < 0.05), while there was not significantly different between two experimental groups (P > 0.05). Moreover the marrow biopsy showed that bone marrow hyperplasia was active in two intervention study groups with some megaryocytes in the medullary cavity of bone while bone marrow hyperplasia was low in the control group without any megaryocyte in the medullary cavity of bone. CONCLUSION: The Sarcandra glabra Thunb had obvious activity in treatment to therombocytopenia and can prevent thrombocytopenia by 5-FU.