| 伯氏疏螺旋体5S～23SrRNA和D剃基因在浙江省鼠标本检测中的应用 |
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| 引用本文： | 姜理平,韦亦成,张磊,应凯满,占利,陆群英.伯氏疏螺旋体5S～23SrRNA和D剃基因在浙江省鼠标本检测中的应用[J].中国媒介生物学及控制杂志,2014(3):249-253. |
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| 作者姓名： | 姜理平 韦亦成 张磊 应凯满 占利 陆群英 |
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| 作者单位： | [1]浙江省疾病预防控制中心病原微生物所,杭州310051 [2]磐安县疾病预防控制中心,杭州310051 |
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| 摘 要： | 目的探索5S～23SrRNA和ospA基因序列在伯氏疏螺旋体检测中的应用，了解浙江省鼠中感染伯氏疏螺旋体状况。方法用PCR方法，检测磐安县100只鼠标本中5S～23SrRNA和ospA基因特异片段。对检测到的阳性结果进行测序。结果检测100只鼠标本，其中3只鼠标本伯氏疏螺旋体的5S～23SrRNA基因片段为阳性，5只鼠标本伯氏疏螺旋体的ospA基因片段为阳性，与参考核酸序列基本一致。结论 利用ospA和5S～23S rRNA基因能从标本中检测到伯氏疏螺旋体。
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| 关 键 词： | 伯氏疏螺旋体 ospA基因 5S～23S rRNA基因 聚合酶链反应 |
Application of 5S-23S rRNA and ospA genes in PCR-based detection of Borrelia burgdorferi in rodent samples of Zhejiang province,China |
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| JIANG Li-ping,WEI Yi-cheng,ZHANG Lei,YING Kai-man,ZHAN Li,LU Qun-ying.Application of 5S-23S rRNA and ospA genes in PCR-based detection of Borrelia burgdorferi in rodent samples of Zhejiang province,China[J].Chinese Journal of Vector Biology and Control,2014(3):249-253. |
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| Authors: | JIANG Li-ping WEI Yi-cheng ZHANG Lei YING Kai-man ZHAN Li LU Qun-ying |
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| Institution: | 1 Zhejiang Center for Disease Control and Prevention, Hangzhou 310051, Zhejiang Province, China; 2 Pan＇ an Center for Disease Control and Prevention) |
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| Abstract: | Objective To evaluate the application of 5S-23S rRNA and outer ospA genes in PCR-based detection of Borrelia burgdorferi and to investigate the infection with B. burgdorferi in rodents of Zhejiang province, China. Methods PCR was used to amplify 5S-23S rRNA and ospA gene fragments from 100 mice collected from different areas of Pan＇ an county. PCR products were purified and sequenced. Results Of the 100 mice investigated, 3 were positive for 5S-23S rRNA gene of B. burgdorferi and 5 for ospA gene of B. burgdorferi. The sequences of PCR products were found with high homology to the reference genes. Conclusion B. burgdorferi can be detected from rodent samples by PCR amplification of 5S-23S rRNA and ospA genes. |
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| Keywords: | Borrelia burgdorferi ospA gene 5S-23S rRNA gene Polymerase chain reaction |
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