宫颈癌组织中Survivin,Bcl-2,P53蛋白的表达

目的:探讨宫颈癌组织中Survivin、Bcl -2及P53蛋白的表达及意义。方法:采用SP免疫组织化学方法, 检测10例正常宫颈组织,18例宫颈不典型增生组织,21例宫颈原位癌组织,69例宫颈浸润癌组织中Survivin、bcl -2 及P53蛋白的表达。结果:正常宫颈组织中Survivin、bcl -2、P53蛋白均不表达;宫颈不典型增生、原位癌及浸润癌组 织中,Survivin阳性表达率分别为50.0%,52.4%和91.3%;Bcl 2阳性表达率分别为44.4%,57.1%和63.8%;P53 阳性表达率分别为0,28.6%和59.4%。3者阳性表达率均随病变程度加深而逐渐升高(P均<0.05)。宫颈癌组 织中Survivin与Bcl -2及P53表达均密切相关(rs分别为0.409,0.373,P均<0.05)。结论:Survivin阳性表达出现 在宫颈癌恶性转化的早期,其与宫颈癌组织中Bcl -2及P53的异常表达密切相关。

Expression of Survivin,Bcl-2,P53 protein in human cervical carcinoma tissue

Aim: To study the expression of Survivin,Bcl-2, and P53 protein in cervical carcinoma tissue. Methods: Using streptavidin-biotin peroxidase(SP) method,the expression of Survivin,Bcl-2, and P53 protein in tissue of 10 cases of normal controls, 18 cases of atypical hyperplasia,21 cases of carcinoma in situ,and 69 cases of cervical invasive carcinoma were examined.Results:The 3 proteins did not express in normal cervical epithelium tissue.The positive rates of Survivin,Bcl-2,and P53 protein all increased from atypical hyperplasia,carcinoma in situ to cervical invasive carcinoma.The positive rate of Survivin was associated with those of Bcl-2 and P53 protein (r_s=0.409,0.373,P<0.05).Conclusion: Survivin, alone or in cooperation with bcl-2 and P53, may participate in onset and progression of cervical carcinoma.