uPAR在大鼠睾丸第一精子发生波中的表达变化

目的:探讨uPAR在大鼠精子发生中的作用。方法:取出生后d0、d5、d10、d15、d21、d28、d35、d42、d49、d56SD大鼠睾丸组织,采用实时荧光定量分析法检测各年龄组大鼠尿激酶受体(uPAR)mRNA表达,并用Western印迹法检测各年龄组大鼠uPAR蛋白表达。结果:大鼠睾丸组织uPARmRNA表达与蛋白表达有相似的趋势:刚出生时表达水平较高,后逐渐下降,约在d15达最低,到d28时开始增加,到d35时到达高峰,随后d42下降,之后保持一定的水平。双变量回归相关分析显示,uPARmRNA表达与蛋白表达为中度正相关。结论:大鼠睾丸uPAR表达在第一精子发生波中出现2个高峰,第一高峰在刚出生时,说明uPAR与精子发生的启动有着密切联系。第二高峰是在出生第5周,这说明uPAR可能参与精子排放过程中的组织重塑及精子变态过程。

Expression of uPAR in Rat Testis at First Wave of Spermatogenesis

Objective: To explore the role of urinary-type plasminogen activator receptor(uPAR) system in rat spermatogenesis. Methods: The day of birth was designated as postnatal d 0, the male rats were divided into 10 groups according to different postnatal stages, that is, d 0, d 5, d 10, d 15, d 21, d 28, d 35, d 42, d 49, d 56. The gene and protein expression of uPAR in rat testis was quantitated by Real-time polymerase chain reaction (PCR) and western blot methods respectively. Results: Gene and protein expression of uPAR had similar tendency in testis of rats at first wave of spermatogenesis. Express level of uPAR was high relatively at postnatal d 0, declined to a lowest level at postnatal d 15, then increased obviously at postnatal d 28 and reached peak at postnatal d 35, then declines at postnatal d 42 and retained low afterwards. Regression and correlation analysis showed that there was a moderately positive correlation between the gene and protein expression levels of uPAR. Conclusion: Expression of uPAR exhibited two peaks in testis of rats at first wave of spermatogenesis: first peak was at short after birth, when gonacyte migrated to the basilar part of seminiferous tubules and was crucial to initiate spermatogenesis, which indicated uPAR had intimate relationship with onset of spermatogenesis; second peak was at postnatal d 35 when round spermatids transformed to elongate spermatids and began to spermiation, which indicated uPAR may be involved in spermiation and/or spermiogenesis.