力竭运动后24 h模型大鼠肾脏结构和炎症因子对黑加仑提取物干预的反应 |
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引用本文: | 刘霞,;蒋嘉烨,;陆海英,;顾翠英,;蔡美琴. 力竭运动后24 h模型大鼠肾脏结构和炎症因子对黑加仑提取物干预的反应[J]. 中国临床康复, 2014, 0(49): 8020-8025 |
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作者姓名: | 刘霞, 蒋嘉烨, 陆海英, 顾翠英, 蔡美琴 |
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作者单位: | [1] 上海中医药大学 公共健康学院,上海市201203; [2] 上海中医药大学 教学实验中心,上海市201203; [3]上海中医药大学 护理学院,上海市201203; [4]上海交通大学医学院,上海市200025 |
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基金项目: | 上海市教委预算内项目(A1-20140315) 感谢上海中医药大学科技实验中心陆熊老师、林敏老师、袁琳老师和胡娜老师对病理切片及观察给予的指导!感谢本科生张婷、许清参与实验 |
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摘 要: | 背景:急性运动后肾脏组织易出现运动性肾缺血再灌注现象,自由基生成增加,发生炎症反应,导致肾功能异常。目的:观察黑加仑提取物对力竭运动后24 h大鼠肾脏结构、肿瘤坏死因子α和核因子κB表达的影响。方法:30只雄性Wistar健康大鼠随机分为3组,每组10只,黑加仑提取物组给予0.44 g/kg黑加仑提取物灌胃,安静对照组、力竭运动后24 h组灌服同等体积的蒸馏水,连续灌胃6周。力竭运动后24 h组和黑加仑提取物组于末次灌胃后进行无负重游泳运动直至力竭疲劳,于24 h后取材,电镜和光镜观察肾脏组织形态和超微结构变化,免疫组化法检测肿瘤坏死因子α蛋白和核因子κB蛋白表达,RT-PCR检测肿瘤坏死因子α mRNA和核因子κB mRNA的表达。结果与结论:与安静对照组比较,力竭运动后24 h组中肾脏核因子κB蛋白和肿瘤坏死因子α蛋白高表达,核因子κB mRNA和肿瘤坏死因子α mRNA表达水平极显著增加(P 〈0.01);与力竭运动后24 h组比较,黑加仑提取物组中肾脏核因子κB蛋白和肿瘤坏死因子α蛋白低表达,核因子κB mRNA表达水平显著性降低(P 〈0.05);肿瘤坏死因子α mRNA表达水平极显著性下降(P〈0.01)。力竭运动后24 h组大鼠肾脏发生明显的形态变化和超微结构损伤,黑加仑提取物组大鼠肾脏结构趋于正常。结果表明黑加仑提取物可修复力竭运动后24 h肾脏组织结构,减少炎症因子的表达,防止肾脏发生炎症性损伤。
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关 键 词: | 实验动物 组织工程 黑加仑提取物 肿瘤坏死因子α 肾脏结构 |
Response of the structure of rat kidney and inflammatory factors to Blackcurrant Extract at 24 hours after exhaustive exercise |
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Affiliation: | Liu Xia, Jiang Jia-ye, Lu Hai-ying, Gu Cui-ying, Cai Mei-qin (1School of Public Health, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; 2Experiment Center for Teaching and Learning, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; 3School of Nursing, Shanghai University of Traditional ChJnese Medicine, Shanghai 201203, China; 4School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, China) |
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Abstract: | BACKGROUND:The kidney tissues easily affected exercise ischemia reperfusion, increased free radicals and inflammation, resulted in abnormal renal function after acute exercise. OBJECTIVE:To observe the influence of Blackcurrant Extract on the structure of kidney and expression of tumor necrosis factor-α and nuclear factor-κB at 24 hours after exhaustiveexercise. METHODS:A total of 30 male Wistar rats were randomly divided into three groups (n=10). Rats in the Blackcurrant Extract group were intragastricaly administered 0.44 g/kg Blackcurrant Extract. Rats in the quietness control group and 24-hour exhaustive exercise group were intragastricaly given an equal volume of distiled water for 6 consecutive weeks. Rats in the 24-hour exhaustive exercise group and Blackcurrant Extract group received no swimming motion until exhaustion fatigue after final intragastric administration. Twenty-four hours later, samples were obtained. Kidney tissue morphology and ultrastructure were observed by electron microscopy and light microscopy. Protein expression of tumor necrosis factor-α and nuclear factor-κB was detected using immunohistochemistry. Tumor necrosis factor-αmRNA and nuclear factor-κB mRNA expression was detected using RT-PCR. RESULTS AND CONCLUSION:Compared with the quietness control group, tumor necrosis factor-α protein and nuclear factor-κB protein expression in the kidney was higher in the 24-hour exhaustive exercise group, and tumor necrosis factor-α mRNA and nuclear factor-κB mRNA expression was significantly increased (P 〈 0.01). Compared with the 24-hour exhaustive exercise group, tumor necrosis factor-α protein and nuclear factor-κB protein expression was lower in the Blackcurrant Extract group, and nuclear factor-κB mRNA expression was significantly decreased (P 〈 0.05); tumor necrosis factor-α mRNA expression was significantly reduced (P 〈 0.01). Kidney in the 24-hour exhaustive exercise group showed obvious morphological changes and ultrastructural dam |
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Keywords: | ribes exercise kidney NF-kappa B tumor necrosis factor-alpha |
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