HPLC法测定茵芍平肝颗粒中栀子苷芍药苷和橙皮苷的含量

目的建立HPLC法测定茵芍平肝颗粒中栀子苷、芍药苷和橙皮苷的含量。方法采用Agilent SB-AQ（250mm×4.6 mm,5μm）色谱柱;流动相：乙腈-水（17∶83）,流速0.8 ml·min-1,检测波长230 nm,柱温30℃,进样量：20μl。结果栀子苷、芍药苷、橙皮苷分别在3.02~30.18、1.00~10.00、1.25~12.00μg·ml-1（n=7）浓度范围内与峰面积线性关系良好;平均回收率分别为99.81%、99.95%、99.55%（n=9）。结论该方法操作简便、准确、专属性好,可作为茵芍平肝颗粒质量控制的方法。

Simultaneous Determination of Geniposide Paeoniflorin and Hesperidin in Yinshao Pinggan Granules by HPLC

Objective To establish an HPLC method for simultaneous determination of geniposide,paeoniflorin and hesperidin in Yinshao Pinggan granules.Methods HPLC analysis was carried out on an Agilent SB-AQ（250 mm × 4.6 mm,5 μm） column.The mobile phase was acetonitrile-water（17∶ 83）.The detection wavelength was 230 nm,the column temperature 30 ℃,and the injection volume 20 μl.Results Geniposide,paeoniflorin and hesperidin showed good linearity in the range of 3.02-30.18,1.00-10.00 and 1.25-12.00 μg·ml-1,respectively.The average recovery of geniposide was 99.81%,that of paeoniflorin was 99.95%,and that of hesperidin was99.55%（n = 9）.Conclusion This method is simple with high accuracy and specificity,which can be used for quality control of Yinshao Pinggan granules.