仿生组装淫羊藿苷-壳聚糖/羟基磷灰石骨修复材料的体外释药行为

背景：淫羊藿苷性质稳定，可促进成骨细胞的增殖，可作为一种骨诱导活性因子用于骨组织工程的研究。目的：探讨淫羊藿苷-壳聚糖/羟基磷灰石骨修复材料的体外释药行为。方法：将载药量分别为10^-7，10^-6，10^-5 mol的淫羊藿苷-壳聚糖/羟基磷灰石材料置于盛有PBS的密闭玻璃离心管中，37℃恒温振荡，分别于1，2，3，10，15，20，30，60，90 d定时收集全部溶液进行超高效液相色谱检测。通过精密度实验、重复性实验、加样回收率实验、稳定性实验考查实验方法的精密度、灵敏度和淫羊藿苷的稳定性，通过标准曲线换算供试品每次释药量，并将其进行累积绘制成释药曲线，采用Logarithmic模型对释药行为进行曲线拟合。结果与结论：实验方法的精密度、准确度与重复性良好；淫羊藿苷性质稳定，可在室温避光的环境下保存至少90 d；在1-2000 ng质量范围内，淫羊藿苷的色谱峰面积与进样量之间呈良好的线性关系。释药初期（0-3 d），药物从支架材料中突释，约达载药量的25%，而后释药速度迅速下降，至第20天有40%-60%的药物释出，之后以低速持续释放，90 d后仍有部分药物存留于支架材料中。3种载药量淫羊藿苷-壳聚糖/羟基磷灰石材料的释药行为遵循一级方程模型，所有模型均有统计学意义（P=0）。表明仿生组装淫羊藿苷-壳聚糖/羟基磷灰石骨修复材料对淫羊藿苷具有良好的控释效果，其体外释药行为符合一级方程。

Release behavior of icariin-chitosan/hydroxyapatite scaffoldsin vitro

BACKGROUND： Icariin has stable nature, which can promote the proliferation of osteoblasts. As a result, it can be used as a new kind of bone induction activity factor for bone tissue engineering.
OBJECTIVE： To study the icariin release behavior of icariin-chitosan/hydroxyapatite scaffolds in vitro.
METHODS： Icariin-chitosan/hydroxyapatite scaffolds （10^-7, 10^-6, 10^-5 mol） were soaked in PBS at 37℃ and shaken gently. The samples collected at 1, 2, 3, 5, 10, 15, 20, 30, 60 and 90 days were analyzed by ultra performance liquid chromatography. The precision and sensitivity of ultra performance liquid chromatography were evaluated by precision test, repeatability test, icariin recovery test and stability test. Icariin released from scaffolds was calculated according to the standard curve and the percentage of icariin released was accumulated to draw a release curve which was fitted based on the Logarithmic model.
RESULTS AND CONCLUSION： The precision, accuracy and repeatability of the experimental method were
good, and icariin exhibited a good stability that could be saved darkly at room temperature at least for 90 days. There was a good linear relationship between icariin dose （1-2 000 ng） and chromatographic peak area. At day 1 to day 3, approximately 25% icariin was released; then the release speed was decreased and 40%-60% icariin was released by the end of 20 days. After 90 days, there was still a certain amount of icariin remained in the scaffolds. The release behavior of icariin-chitosan/hydroxyapatite followed the first-order equation, and there were significant difference among all the models （P = 0）. These findings indicate that icariin-chitosan/hydroxyapatite scaffolds have a good control effects on icariin release, and the icariin release behavior can be fitted with the first-order equation.