HPLC法测定复方甲氧沙林乳膏中甲氧沙林和醋酸氯已定的含量

目的建立HPLC法同时测定复方甲氧沙林乳膏中甲氧沙林和醋酸氯已定的含量。方法色谱柱Thermo,ODS-2HYPERSIL（250 mm×4.6 mm）;流动相：甲醇-水（70∶30）;流速：1.0 ml·min-1;检测器：DAD检测器;检测波长：249 nm和259 nm;柱温：30℃。结果醋酸氯已定和甲氧沙林线性范围分别为0.1032~2.064μg（r=0.9998）和0.0993~1.986μg（r=0.9999）。平均加样回收率分别为97.7%（RSD=1.2%）和94.4%（RSD=1.6%）。结论本法操作简便、准确、重复性好,回收率高,可用于复方甲氧沙林乳膏的质量控制。

Determination of Content of Methoxsalen and Chlorhexidine Acetate in Compound Methoxsalen Cream by HPLC

Objective To establish an HPLC method for determination of methoxsalen and chlorhexidine acetate in compound methoxsalen cream.Methods The separation was performed on a Thermo,ODS-2HYPERSIL（250 mm × 4.6 mm） column with a moble phase of methanol-water（70∶ 30） at a flow rate of 1.0 ml·min-1.The DAD detection wavelength was 249 nm and 259 nm respectively.The column temperature was 30 ℃.Results The calibration curves of chlorhexidine acetate and methoxsalen were linear in the range of 0.1032-2.064 μg（r = 0.9998）and 0.0993-1.986 μg（r = 0.9999） respectively.The average recovery of chlorhexidine acetate and methoxsalen was 97.7%（RSD = 1.2%.） and 94.4%（RSD = 1.6%） respectively.Conclusion This method is simple,accurate and repeatable,which can be used for quality control of compound methoxsalen cream.