| HPLC法测定远志合剂中细叶远志皂苷的含量 |
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| 引用本文： | 刘雪梅,;庄珊珊,;刘志宏,;黄爱文,;宋洪涛. HPLC法测定远志合剂中细叶远志皂苷的含量[J]. 解放军药学学报, 2014, 0(6): 522-524 |
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| 作者姓名： | 刘雪梅, 庄珊珊, 刘志宏, 黄爱文, 宋洪涛 |
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| 作者单位： | [1]南京军区福州总医院药学科,福建福州350025; [2]福建中医药大学药学院,福建福州350108 |
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| 基金项目： | 军队医疗机构制剂标准提高科研专项课题,No.132J01 |
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| 摘 要： | 目的建立测定远志合剂中细叶远志皂苷含量的方法。方法采用HPLC法,色谱柱为Kromasil C18（150mm×4.6 mm,5μm）,以乙腈-0.1%磷酸溶液（33∶67）为流动相,检测波长为210 nm,流速1 ml·min-1,进样量为10μl。结果细叶远志皂苷检测浓度的线性范围为5.0~90.0μg·ml-1（r=0.9999）;平均加样回收率为98.58%,RSD为1.6%（n=9）。结论本方法简便、准确、重复性良好,适用于远志合剂的质量控制。
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| 关 键 词： | 远志合剂 细叶远志皂苷 HPLC 含量测定 |
Content Determination of Tenuifolin in Yuanzhi Mixtura by HPLC |
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| Affiliation: | LIU Xue-mei,ZHUANG Shan-shan,LIU Zhi-hong,HUANG Ai-wen,SONG Hong-tao(1.Department of Pharmacy, Fuzhou General Hospital of Nanjing Military Region, Fuzhou 350025, China ; 2.Department of Pharmacy,Fujian College of Traditional Chinese Medicine,Fuzhou 350108,China) |
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| Abstract: | Objective To establish a method for content determination of tenuifolin in Yuanzhi mixtura.Methods HPLC method was adopted.The column was Kromasil C18（150 mm × 4.6 mm,5 μm）.The mobile phase consisted of acetonitrile-0.1% orthophosphoric acid（33∶ 67） at a flow rate of 1 ml·min-1.The UV detection wavelength was at 210 nm.The injection volume was 10 μl.Results The standard curve of tenuifolin was linear over the range of 5.0-90.0 μg·ml-1（r = 0.9999） with an average recovery of 98.58%（RSD = 1.6%,n = 9）.Conclusion The method is accurate,reliable and reproducible,so it can be used for quality standard control of yuanzhi mixtura. |
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| Keywords: | Yuanzhi mixtura tenuifolin HPLC content determination |
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