诱导多能干细胞的研究进展及其瓶颈

背景：随着研究的深入，诱导多能干细胞被应用于疾病模型建立、药物筛选和再生医学等多个研究领域，尤其是应用于神经系统疾病的研究。 目的：总结诱导多能干细胞目前的进展并结合相关领域的最新研究提出问题和展望。 方法：第一作者检索2006年1月至2014年9月PubMed数据库中有关诱导多能干细胞的相关文献，检索词为“iPS，induced pluripotent stem cel”，包括综述、临床研究和基础研究，最终纳入60篇文献。 结果与结论：2006年Yamanaka团队的诱导多能干细胞问世至2012年诺贝尔奖的颁发，短短6年间诱导多能干细胞相关的研究不断取得突破。诱导多能干细胞研究在疾病模型、药物筛选和再生医学等方面拥有广阔的前景，目前存在包括重编程方式、细胞稳定性、临床转化等一系列问题亟待解决，仍需进一步研究深化。

Research progress of induced pluripotent stem cells and its bottleneck

BACKGROUND：With the development of the research, induced pluripotent stem cel s are applied to the build of disease model, drug screening, regenerative medicine, and many other research fields, and have made significant achievements, especial y in the study of nervous system diseases. OBJECTIVE：To summarize the recent development of induced pluripotent stem cel s and to raise problems and prospects based on the latest research in this field. METHODS：The first author searched the PubMed database for articles about the induced pluripotent stem cel s, including reviews, clinical research and basic research, published from January 2006 to September 2014. The keywords were“iPS, induced pluripotent stem cel”, and final y 60 articles were included in result analysis. RESULTS AND CONCLUSION：Induced pluripotent stem cel research continues to make breakthrough from its discovery by Yamanaka’s team in 2006 to winning Nobel Prize in 2012. Induced pluripotent stem cel research has broad prospects in the disease model construction, drug screening and regenerative medicine. Currently, problems such as reprogramming methods, cel stability, and clinical transformation stil need to be solved, and further researches are necessary.