| UHPLC法同时测定复方丁香油涂剂中丁香酚和樟脑的含量 |
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| 引用本文： | 马金刚,;危雪平,;王鹏远,;郭飞燕,;王晓娟.UHPLC法同时测定复方丁香油涂剂中丁香酚和樟脑的含量[J].解放军药学学报,2014(5):423-424. |
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| 作者姓名： | 马金刚 ;危雪平 ;王鹏远 ;郭飞燕 ;王晓娟 |
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| 作者单位： | [1]兰州军区联勤部药品仪器检验所,甘肃兰州730050; [2]第四军医大学口腔医院药剂科,陕西西安710032 |
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| 基金项目： | 军队医疗机构制剂标准提高计划,No.13ZJZ08 |
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| 摘 要： | 目的建立UHPLC法同时测定复方丁香油涂剂中丁香酚和樟脑的含量。方法采用Agilent Zorbax SB-C18（2.1 mm×50 mm,1.8μm）色谱柱,流动相：甲醇-0.1%甲酸水溶液（58∶42）,检测波长：285 nm,流速：0.2 ml·min^-1,柱温：25℃。结果丁香酚、樟脑分别在27.48~274.80μg·ml^-1（r=0.9995）和0.38~2.28 mg·ml^-1（r=0.9998）范围内与峰面积线性关系良好;平均加样回收率分别为99.98%和100.12%,RSD分别为1.14%和0.75%（n=9）。结论本方法操作快速,结果准确,专属性强,可用于复方丁香油涂剂中丁香酚和樟脑的含量测定。
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| 关 键 词： | UHPLC 复方丁香油涂剂 丁香酚 樟脑 含量测定 |
Simultaneous Determination of Eugenol and Camphor in Compound Clove Oil Paint by UHPLC |
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| Institution: | MA Jin-gang, WEI Xue-ping, WANG Peng-yuan, GUO Fei-yan, WANG Xiao-juan(1Institute for Drug and Instrument Control of Lanzhou Military Command,Lanzhou 730050 ,China;2Department of Pharmacy,Stomatological Hospital of the Fourth Military Medical University,Xi＇an 710032 ,China) |
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| Abstract: | Objective To establish a UHPLC method for simultaneous determination of eugenol and camphor in compound clove oil paint. Methods The UHPLC procedure was performed on the chromatographic column of Agi- lent Zorbax SB-C18 （2.1 mm×50 mm,1.8μm） at 25 ℃. The mobile phase was methanol： 0.1% formic acid solution（58： 42）. The flow rate was 0.2 ml .min- 1 and detection wavelength was 285 nm. Results Good linear correla- tions were observed within the range of 27.48 - 274.80 μg .ml^-1 for eugenol （ r = 0. 9995 ） and 0.38 - 2.28 mg. ml^-1 for camphor （ r =0.9998）. The average recovery（ n =9）was 99.98% （RSD = 1.14 % ） and 100.12% （ RSD = 0.75 % ）. Conclusion This method is quick, accurate, specific, and can be applied to the content deter- mination of eugenol and camphor in compound clove oil paint. |
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| Keywords: | UHPLC compound clove oil paint eugenol camphor content determination |
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