Specific in Vitro Proliferative Immune Responses and Lymphokine Production in Ethiopian Children with and without Tuberculosis |
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Authors: | M Kori V Barak E Leibovitz Y Altman A Eliraz ZT Handzel |
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Institution: | (1) Kaplan Hospital, Kaplan Medical Center, IL-76100 Rehovot, Israel; Phone: +972-89441571, Fax: +972-8952369, IL;(2) Hadassah Medical Center, Ein Karem, Jerusalem, Israel, IL;(3) Soroka Medical Center, Beer-Sheba, Israel, IL |
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Abstract: | Summary
We investigated the profile of some in vitro parameters of cellular immune responses in non-HIV-infected Ethiopian children and young adults with and without tuberculosis
(TB) as compared to healthy Ethiopian and non-Ethiopian controls. The in vitro proliferative responses of peripheral blood mononuclear cells (PBMC) to purified protein derivative (PPD) were determined
in 15 Ethiopian children and young adults with TB, 12 healthy Ethiopian children who were contacts of TB patients, 20 Ethiopian
children without contact with TB and ten non-Ethiopian controls. All TB patients and contacts had a positive Mantoux skin
test. The PBMC proliverative response to PPD of the Ethiopian children with TB was significantly higher than that of the Ethiopian
children without TB, while all Ethiopian children demonstrated stronger proliferative response as compared to non-Ethiopian
healthy controls. Interleukin 2 (IL-2), interferon gamma (IFN-γ), interleukin 4 (IL-4) and interleukin 6 (IL-6) were measured
by ELISA assays performed on the supernatant of PPD-stimulated and non-stimulated PBMC cultures of seven Ethiopian children
with TB, ten Ethiopian children without TB and eight non-Ethiopian controls. IFN-γ and IL-4 were undetectable and IL-2 levels
in unstimulated supernatants were low in all groups. PPD stimulation induced a significant rise in IL-2 levels in Ethiopians
with TB as compared to all other groups. There was no increase above baseline in IL-6 levels in any group studied. Conclusions:
Ethiopian children with TB exhibit a strong cellular immune response as expressed by Mantoux tests and lack of stimulation
of IL-4 and IL-6 production. This pattern suggests a Th1 type effective cellular immune response to mycobacteria in a cohort
of young Ethiopians with TB.
Received: December 3, 1998 · Revision accepted: December 4, 1999 |
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Keywords: | Tuberculosis Immune responses Lymphokines |
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