Alteration in systemic markers of oxidative and antioxidative status in Tunisian patients with asthma: relationships with clinical severity and airflow limitation |
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| Authors: | Amel ben Anes Hela ben Nasr Hamadi Fetoui Sarra Bchir Hassiba Chahdoura Saloua Yacoub |
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| Affiliation: | 1. Unité de Recherche UR12ES06, Physiologie de l’Exercice et Physiopathologie, de l’Intégré au Moléculaire, Biologie, Médecine et Santé, Université de Sousse, Sousse, Tunisia,;2. Faculté des Sciences de Bizerte, Université de Carthage, Bizerte, Tunisia,;3. Unité de Recherche UR12ES06, Physiologie de l’Exercice et Physiopathologie, de l’Intégré au Moléculaire, Biologie, Médecine et Santé, Université de Sousse, Sousse, Tunisia,;4. Faculté des Sciences de Sfax, Unité de Recherche de Toxicologie-Microbiologie Environnementale et Santé (UR/11 ES70), Sfax, Tunisia,;5. Génome, Diagnostic Immunitaire et valorisation, Unité de Recherche 03/UR/09-01, Institut Supérieur de Biotechnologie de Monastir, Université de Monastir, Monastir, Tunisia,;6. Unité de Recherche, UR06SP05, Centre Régional de Transfusion Sanguine, CHU Farhat Hached, Sousse-Tunisia, |
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| Abstract: | Objective: This study aims to determine the systemic oxidant-antioxidant status in Tunisian patients with asthma. Methods: We evaluated the levels of malondialdehyde (MDA) as thiobarbituric acid complexes, total protein carbonyls (PCs) and advanced oxidation protein products (AOPP). The levels of total thiols, protein sulfhydryls, glutathione (GSH), together with hydrogen peroxide, ascorbic acid, iron and total antioxidant status (TAS) were colorimetrically estimated. Glutathione peroxidase (GSH-Px), catalase (CAT) and superoxide dismutase (SOD) activities were assessed in plasma and erythrocytes by spectrophotometry. We also determined the levels of nitric oxide (NO) and peroxynitrite in plasma from asthmatic patients and healthy controls. The volume of fractionated exhaled NO (FeNO) was evaluated by the Medisoft HypAir method. Estimation of DNA damage was determined using the comet assay. Results: Asthmatic patients showed increased levels of MDA in comparison to healthy controls (p?p?=?0.79) and AOPP (p?=?0.98). Patients with asthma also had significantly lower levels of total thiols (355.9?±?15.72 versus 667.9?±?22.65, p?p?p?p?p?=?0.06 and p?=?0.55). In addition, ascorbic acid and nitric oxide levels were decreased in asthmatics in comparison to controls (p?Conclusions: Our findings highlight that oxidative stress and defective anti-oxidative status are major alterations in Tunisian patients with asthma. |
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| Keywords: | Asthma airflow limitation antioxidants glutathione oxidative stress |
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