Evaluation of DNA damage induced by norcantharidin in human cultured lymphocytes |
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| Authors: | Omar F. Khabour Fatima M. Enaya Karem Alzoubi Sayer I. Al-Azzam |
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| Affiliation: | 1. Department of Medical Laboratory Sciences, Jordan University of Science and Technology, Irbid, Jordan,;2. Biology Department, Faculty of Science, Taibah University, Almedina, Saudi Arabia, and khabour@just.edu.jo;4. Department of Medical Laboratory Sciences, Jordan University of Science and Technology, Irbid, Jordan,;5. Department of Clinical Pharmacy, Jordan University of Science and Technology, Irbid, Jordan |
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| Abstract: | Norcantharidin (NCTD) is currently used in the treatment of several cancers such as leukemia, melanoma and hepatoma. The mechanism of action of NCTD is suggested to involve induction of apoptosis of cancer cells via production of reactive oxygen species. In this study, the genotoxic effect of different concentrations of NCTD (1, 10 and 20?μm) in human lymphocytes was investigated using sister chromatid exchanges (SCEs) and chromosomal aberrations (CAs) assays. The results revealed that NCTD significantly increased the rate of SCEs (p?p?p?>?0.05). In addition, no significant differences were detected in the mitotic index or proliferative index at examined doses (up to 20?μm). In conclusion, NCTD is genotoxic to human cultured lymphocytes as measured by SCE assay. |
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| Keywords: | Norcantharidin (CAS number 29745-04-8) DNA damage human lymphocytes |
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