逐痰通络汤对局灶性脑缺血大鼠凝血纤溶系统的影响

目的：观察逐痰通络汤对局灶性脑缺血大鼠凝血纤溶系统的影响。方法：清洁级雄性SD大鼠18只,随机分为假手术组（K组）、模型组（C组）和逐痰通络汤组（Z组）,每组各6只。应用线栓法建立大脑中动脉栓塞（MCAO）模型。造模后第7天观察各组大鼠神经功能缺损并评分,脑组织TTC染色并测定梗死体积,以发色底物法测定血浆组织型纤溶酶原激活物（t-PA）、组织型纤溶酶原激活抑制物（PAI）、纤溶酶原（PLG）活性,ELISA法测定血浆D-二聚体（DD）活性。结果：C组、Z组均出现不同程度神经功能缺损和梗死灶。与C组相比,Z组神经功能缺损较轻、梗死体积较小（P〈0.05）。C组、Z组PLG、PAI、DD活性显著高于K组,t-PA活性显著低于K组（P〈0.01）。Z组PLG、PAI、DD活性低于C组（P〈0.05,P〈0.01）,t-PA活性高于C组（P〈0.05）。结论：增强血浆纤溶活性,纠正凝血纤溶系统失衡,可能是逐痰通络汤治疗脑缺血的机制。

Effect of Zhutan Tongluo Tang on the coagulation and fibrinolysis of the focal cerebral ischemia in rats

Objective：To observe the effect of Zhutan Tongluo Tang on the coagulation and fibrinolysis of focal cerebral ischemia in rats.Methods：Eighteen Sprague-Dawley male rats were divided randomly into 3 groups：sham operation group（K）,model group（C） and Zhutan Tongluo Tang group（Z）.The middle cerebral artery occlusion（MCAO） model was established with the thread-tying method.At 7 days after operationt,he scores of neural functional defect were measured,the tissues of brain were stained with TTC to measure infarct area,the activity of t-PA,PAI and PLG was measured using CLIA.The activity of DD was measured using ELISA.Results：Rats of C and D group had neural functional defect and infarct area with different degree.Compared with C group,the neural functional defect of Z group was lighter and the infarct area was smaller（P 0.05）.The activity of PLG,PAI and DD in C and D groups was significant higher than that of K group but t-PA was significant lower（P 0.01）T.he activity of PLG,PAI and DD in Z group was lower than that of C group（P 0.05,P 0.01）but t-PA was higher（P 0.05）.Conclusion：Promot-ing activity of fibrinolysis system and correcting disequilibrium of coagulation and fibrinolysis system are probably mechanism that Zhutan Tongluo Tang treats ischemia.