鼠脑缺血性损伤早期海马区PAF和LPO含量的动态观察及其意义

目的：运用脑缺血再灌注模型，探索血小板活化因子及自由基在缺血性脑损伤中的地位及意义，方法：分别应用放免法及ＴＢＡ法法测定大鼠海马组织中ＰＡＦ与ＬＰＯ含量。结果：缺血２０ｍｉｎ后灌注０ｍｉｎ组及６０ｍｉｎ组ＰＡＦ含量均显著高于手术对照组，而再灌注２４０ｍｉｎ组与假手术对照组比较已无显著差异。相应ＬＰＯ含量早期随再灌注时间延长逐渐升高。结论：ＰＡＦ可能主要能与脑缺血再灌注缺血期的病理损伤机制，而自由基

The dynamic observation of PAF and LPO levels of rat hippocampus on the cerebral ischemic reperfusion damage

: In order to study the action and significance of platelet-activating factor (PAF)andLipid peroxidex(LPO) on the cerebral ischemic damage,the levels in rat hipppocampus were observed dynamically.Methods: 26 mature Wistar rats were randomly divided into four groups: Sham-operated control group, O-minute,6o-minute and 240-minute reperfusion groups. With the 4-vessel occlusion (4VO )model, the rats were suffered fromischemia for 20 minutes, the reperfusion was allowed for 0. 60' 240 minutes before decapitation. Sham-operatedcontrol rats were the same except for the clamping vessels. Radioimmunoassay and thiobarbituric acid reaction wereapplied to measure tlle PAF and LPO concentrations of rat hippocampus recpectively. Results: The PAFconcentrations of O-minute reperfusion group were significantly higher then those of the control group (P<0. 01),but the PAF levels progressively returned to basal values following 240 minute reperfusion. Compared with thecontrol group, the LPO concentrations of O-minute reperfusion group were no significant difference (P> 0. 05),whereas the levels of 60-minute and 240-minute reperfusion groups showed a significant increase (P < 0. 01 ).Conclusion :The results suggested that PAF was involved in the early stages of the cerebral ischemia, especially insimple ischemic period , while the lipid hyperperoxidation might only occure in the reperfusion stage.[