| 衍生化法测定糖尿乐胶囊中尿囊素的含量 |
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| 引用本文： | 尹晓强,代龙,魏永利,张加余.衍生化法测定糖尿乐胶囊中尿囊素的含量[J].中南药学,2008,6(2):203-205. |
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| 作者姓名： | 尹晓强 代龙 魏永利 张加余 |
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| 作者单位： | 1. 广西中医学院,南宁,530001
2. 山东中医药大学,济南,250014 |
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| 摘 要： | 目的建立高效液相色谱法测定糖尿乐胶囊中尿囊素的含量测定方法。方法岛津（SHIMADZU）ODS柱,乙腈-0.1%冰醋酸（三乙胺调pH至5.6～6.0）为流动相,梯度洗脱;检测波长为360nm。结果尿囊素在0.11～0.55μg线性关系良好,平均回收率为99.08%,RSD=1.22%。结论此方法灵敏、准确,可用于尿囊素的含量测定。
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| 关 键 词： | 高效液相色谱法 糖尿乐胶囊 尿囊素 梯度洗脱 |
| 文章编号： | 1672-2981(2008)02-0203-03 |
| 修稿时间： | 2007年10月8日 |
Content determination of Allantion in Tangniaole capsules by derivatization method |
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| YIN Xiao-qiang,DAI Lon,WEI Yong-li,ZHANG Jia-yu.Content determination of Allantion in Tangniaole capsules by derivatization method[J].Central South Pharmacy,2008,6(2):203-205. |
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| Authors: | YIN Xiao-qiang DAI Lon WEI Yong-li ZHANG Jia-yu |
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| Institution: | YIN Xiao-qiang , DAI Long , WEI Yong-li, ZHANG Jia-yu ( Guangxi Traditional Chinese Medical University, Nanning 530001; Shandong University of Traditional Chinese Medicine, Jinan 250355) |
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| Abstract: | Objective To establish a derivatization HPLC method for the determination of the content of allantion in Tangniaole capsules. Methods SHIMADZU ODS C18 column was used. The mobile phase consisted of acetonitrile0. 1% glacial acetic acid （adjusted the pH to 5.6-6.0 by triethanolamine ） and the solvent programming was used. The detective wavelength was 360 nm. Results The linear range of allantion was 0.11-0. 55 μg, and the average recovery was 99.08%, with the RSD 1.22%. Conehtsion The method is sensitive, accurate and can be used for the determination of the content of allantion in Tangniaole capsules. |
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| Keywords: | HPLC Tangniaole capsule allantion gradient elution |
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