麻黄汤及拆方对哮喘小鼠5-脂质氧合酶激活蛋白、白介素4基因的表达和白三烯C4的影响

目的:通过对麻黄汤(Herba Ephedrae decoction,HED)各拆方配伍组对哮喘小鼠5-脂质氧合酶激活蛋白(FLAP)、白介素4(IL-4)基因的表达和白三烯C₄(LTC4)的影响来探讨其配伍规律。方法:建立小鼠卵蛋白哮喘模型,除正常对照组和模型组外其余各组分别灌胃给予麻黄汤及拆方(以麻黄生药剂量2 500 mg.kg^-1)和阳性药地塞米松(2 mg.kg^-1),连续用药7 d后用实时荧光定量PCR方法检测肺组织中5-脂质氧合酶激活蛋白、白介素4mRNA表达的变化,用ELISA方法检测支气管肺泡灌洗液中LTC₄含量。结果:哮喘小鼠肺组织中FLAP,IL-4基因表达水平、支气管肺泡灌洗液(BALF)中LTC₄水平均较正常对照组显著升高。麻黄汤及拆方组可以不同程度抑制小鼠肺组织中FLAP,IL-4基因表达水平,BALF中LTC₄水平。结论:麻黄汤具有明显抗过敏哮喘的作用,拆方分析显示麻黄汤全方效果最佳,从分子水平验证了麻黄汤组方的科学性和合理性。

Effects of among compositions of Herba Ephedrae decoction on genic xpression of 5-lipoxygenase activating protein,IL-4 and leukotriene C4 in asthmatic mice

Objective: To explore the regularity of recipe composition by observing inhibitory effects on the genic expression of 5-lipoxygenase activating protein,IL-4 and the leukotriene C₄ in asthmatic mice.Method: The mice were challenged with OVA and administered ig with the Herba Ephedrae decoction(HED),separated compositions(2 500 mg·kg^-1,calculated by Herba Ephedrae) and dexamethasone(2 mg·kg^-1) respectively once daily for seven days.The real-tme fluorescence quantitative PCR method was employed to measure the contents of FLAP mRNA and IL-4 mRNA expressions in lung and the ELISA method was used to determine the content of LTC₄ in the washing solution of pulmonary alveolus and bronchi.Result: In the lung of asthma mice,the expressions of FLAP and IL-4 and the content of LTC₄ were significantly augmented compared with the control group.The HED and the separated compositions could suppress the expressions of FLAP and IL-4 and LTC₄ release to a great extent in mice.Conclusion: The HED had the remarkable effects of antianaphylaxis asthma and the original formula HED worked best.These results confirmed the rationality and scientific level of HED.