谷胱甘肽转硫酶M1和T1基因型与新疆哈萨克族食管癌的关系

目的 为探讨与致癌物代谢有关的谷胱甘肽转硫酶(GST)Tl和Ml基因多型性与哈萨克族食管癌易感性的关系。方法 采用聚合酶链(PCR)技术，分析新疆食管癌高发民族中GSTMl和GSTTl基因型分布的差异。结果 GSTMl基因在食管癌、癌旁正常粘膜对照组中缺失率分别为41．46％(17／41)、34．15％(14／41)；GSTTl基因缺失率的分别为48．78％(20／41)、51．22％(21／41)，差别均无显性。同时，在食管癌组织的高分化组、中低分化组中GSTTl和GSTMl基因缺失率分别为61．54％(8／13)、42．86％(12／28)、l5．38％(2／l3)、53．57％(14／28)，其中GSTMl基因缺失率在高、中低分化组中差别有统计学意义。结论 提示GSTMl基因多型性与哈萨克族食管癌组织病理分级相关。

Study on the relationship between glutathione S-transferase M1, T1 genotypes and Kazak esophageal cancer

Objective To explore the association between genetic polymorphisms of glutathione S-transferase (GST) M1 and T1 and susceptibility to Kazak population esophageal cancer (EC). Methods A polymerase chain reaction method was used to detect absence of the GSTM1 and GSTT1 genes in genomic DNA in a high-risk ethnic, Xinjiang, China. Results The frequency of the GSTM1-null genotype in cancer cases (41.47%) was not significantly different from that in controls ( 34.15%). Similarly, no statistically significant differences were observed in the frequency of GSTT1-null genotype in cancer cases (48.78%) compared with control (51.22%). However, the frequency of GSTM1 null alleles genotypes in cases with well-differentiated cancer ( 15.38%) showed a significant decrease compared with that in poor-differentiated cancer ( 53.57%) (P<0.05). Conclusions GSTM1-null genotypes may act as risk factor in the development of poor-differentiated esophageal cancer in Kazak population.