| 消毒剂破坏乙、丙型病毒性肝炎血清标志物的初步观察 |
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| 引用本文： | 杨世明,杨枨林,安仁鸣,解华.消毒剂破坏乙、丙型病毒性肝炎血清标志物的初步观察[J].中华流行病学杂志,1995(3). |
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| 作者姓名： | 杨世明 杨枨林 安仁鸣 解华 |
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| 作者单位： | 解放军第四军医大学唐都医院血库 |
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| 摘 要： | 笔者采用氯仿、４０％甲醛溶液、３％双氧水及７５％乙醇与ＨＢｓＡｇ、ＨＢｅＡｇ和抗－ＨＣＶ阳性血清作用５、１５、３０及６０分钟后，用ＥＬＩＳＡ法检测是否转为阴性，以了解消毒剂对上述阳性血清的破坏效果。结果：氯仿对ＨＢｓＡｇ和抗－ＨＣＶ阳性血清破坏效果好；４０％甲醛溶液对ＨＢｓＡｇ、ＨＢｅＡｇ及抗－ＨＣＶ阳性血清均破坏；３％双氧水对ＨＢｅＡｇ有破坏作用，而７５％乙醇对ＨＢｓＡｇ、ＨＢｅＡｇ及抗－ＨＣＶ阳性血清作用上述时间后，检测时仍呈阳性反应。笔者只是初步观察，有关消毒剂的浓度、效果及作用机理有待进一步观察和探讨。
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| 关 键 词： | HBV，Anti－HCV，ELISA |
Prelimimary Observation on the Destructive Effect of Chemical
Disinfectants in serum Markers of Hepatitis B and C |
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| Yang
Shi-ming,Yang Chang-lin,An Ren-ming,et al..Prelimimary Observation on the Destructive Effect of Chemical
Disinfectants in serum Markers of Hepatitis B and C[J].Chinese Journal of Epidemiology,1995(3). |
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| Authors: | Yang
Shi-ming Yang Chang-lin An Ren-ming |
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| Institution: | Yang
Shi-ming,Yang Chang-lin,An Ren-ming,et al.Blood bank,TangDu Hospital,Fourth Military Medical Universty,Xian,710038 |
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| Abstract: | hloroform,40%formaldehyde,3%hydrogen peroxide solution and
75%alcohol were usedto act on hepatitis B surface
antigen(HBeAg),hepatitis B e antigen(HBaAg)and anti-hepatitisC virus
antibody(Anti-HCV)positive serum for 5,15,30 and over 60 minutes.They
were exam-ined by enzyme-linked immunosorbent assay and observed for
the effect.The destructive effect of chloroform on HBsAg and anti-HCV
positive derum was marked.Itwas discovered that 40%formaldehyde
solution destroyed HBsAg,HBeAg,and anti-HCV positiveserm,while
75%alcohol also showed destructive effect on HBsAg,HBeAg and anti-HCV
positive serum. |
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| Keywords: | HBV Anti-HCV ELISA |
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