电针对糖尿病大鼠脑组织游离氨基酸含量的影响

目的观察电针对糖尿病大鼠脑组织游离氨基酸含量的影响。方法采用四氧嘧啶腹腔注射法制备糖尿病大鼠模型,采用高效液相色谱法检测糖尿病大鼠脑组织游离氨基酸含量并观察电针的影响。结果电针组血糖治疗前后差异非常显著(P<0.01),但仍然高于正常组(P<0.001);糖尿病模型组脑内游离氨基酸谷氨酸、天门冬氨酸、甘氨酸、γ-氨基丁酸均较正常组明显增高,(P<0.01)。电针组4种游离氨基酸均较模型组明显降低(P<0.01),其中Gly明显低于正常组(P<0.05)。结论电针能够降低糖尿病大鼠血糖,可以抑制糖尿病大鼠脑组织游离氨基酸含量的升高。

The Influence of Electro-Acupuncture on Free Amino Acid in Brain Tissue of Diabetic Rats

Objective To observe the influence of Electro-Acupuncture on free amino acid in brain tissue of diabetic rats. Method The diabetic rats were induced by the intra-abdominal injection of alloxan (100 mg/kg once a day for two days). Electro-Acupuncture on Bai Hui and Da Zhui together with quick puncturing along Pang Guang meridian on the back, especially the back Shu points of five Zang organs were applied to alloxan-diabetic rats. Blood glucose and free amino acid were investigated after 6 weeks of Electro-Acupuncture treatment. Results The blood glucose of Electro-Acupuncture group was decreased obviously after treatment (P <0.01). The amount of free amino acid such as Glu, Asp, Gly and GABA in the brain of diabetic rats were increased significantly than those of normal group (P <0.01) while the four free amino acid of Electro-Acupuncture group decreased significantly than those of diabetic control group, among them Gly even lower than that of normal group (P <0.05). Conclusion Electro-Acupuncture could bring down blood glucose of the diabetic rats to some extent. Electro-Acupuncture could inhibit the increase of free amino acid in the brain of diabetic rats.