首页 | 本学科首页   官方微博 | 高级检索  
检索        


Purification of murine erythropoietin produced in serum-free cultures of erythroleukemia cells
Authors:Qian  RL; Chin  K; Kim  JK; Chin  HM; Cone  J; Hankins  WD
Abstract:We previously documented that several erythroleukemia cell lines released factors that stimulated erythropoiesis in vivo and in vitro. A simple five-step scheme has been devised that allows purification of this erythropoietic activity to apparent homogeneity. The methods employed included lectin affinity chromatography (wheat germ agglutinin), gel filtration (ultro gel ACA44), ion exchange, hydroxylapatite, and high performance liquid chromatography. Following polyacrylamide gel electrophoresis, biologic activity was recovered in an area corresponding to a molecular weight of 35,000 daltons. Silver staining of a polyacrylamide gel after electrophoresis of our most purified preparation revealed a single band at 35,000 daltons.
Keywords:
点击此处可从《Blood》浏览原始摘要信息
点击此处可从《Blood》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号