阿托伐他汀对高血压大鼠血管纤维化及结缔组织生长因子的影响

目的:探究阿托伐他汀(atorvastatin)对肾血管性高血压血管纤维化及结缔组织生长因子(CTGF)表达的影响。方法:24只SD大鼠随机分为假手术组(N组)、高血压组(RH组)、阿托伐他汀组(他汀组)各8只;将RH组及他汀组大鼠建立二肾一夹肾血管性高血压(2K1C-RH)大鼠模型。术后4周末,他汀组开始以阿托伐他汀(10mg.Kg-1.d-1)灌胃,共8周。治疗前后RT-PCR法检测各组大鼠收缩压、胸主动脉CTGF、纤维连接蛋白(FN)、Ⅲ型胶原(ColⅢ)的表达,并用免疫组化显示CTGF的表达。结果:与N组比较,RH组大鼠胸主动脉CTG-FmRNA、FNmRNA、ColⅢmRNA显著增加(P0.01);他汀组与RH组比较则3项指标明显下调(P0.05)。结论:阿托伐他汀能抑制高血压血管纤维化,可能与下调CTGF的表达有关。

Effects of Atorvastatin on Vascular Fibrosis and Connective Tissue Growth Factor in Renovascular Hypertensive rats

Objective：To investigate the effects of atorvastatin on vascular fibrosis and connective tissue growth factor （CTGF） in renovascular hypertensive （RH） rats. Methods：The following groups of rats were studied： sham group, RH group and atorvastatin （10 mgokg-1od-1）-treated RH group. Systolic blood pressure （SBP） was measured. The mRNA expression of CTGF, FN and ColⅢ was detected by RT-PCR at the 12th week after operation. The expression of CTGF protein was detected by immunohistochemistry. Results： Compared with the normal control group, the mRNA expression of CTGF （P〈0.01）, FN and ColⅢ （P〈0.05） in RH group was increased in the thoracic aorta, and atorvastatin could significantly inhibit vascular fibrosis and the expression of CTGF （P〈0.05）. Conclusion： Atorvastatin could inhibit the development of vascular fibrosis in RH rats, which was probably related to the decreased expression of CTGF.