Raf激酶抑制蛋白对鼻咽癌细胞增殖的影响

目的：探讨Raf激酶抑制蛋白（RKIP）对鼻咽癌细胞增殖的影响。 方法：采用脂质体转染方法将反义RKIP核酸表达质粒pcDNA3.1(-)-asRKIP及空白载体pcDNA3.1(-)分别转染鼻咽癌细胞系6-10B细胞，用Western 印迹检测RKIP表达，建立RKIP表达下调的稳定转染细胞和对照细胞系。采用四甲基偶氮唑蓝（MTT）法、流式细胞术和软琼脂集落形成实验检测RKIP表达下调对鼻咽癌细胞增殖、细胞周期分布和停泊非依赖性生长的影响。结果：建立了RKIP表达下调的6-10B鼻咽癌细胞系；RKIP表达下调促进6-10B细胞增殖和停泊非依赖性生长，并加速其越过G0/G1期。结论：RKIP具有抑制鼻咽癌细胞增殖和停泊非依赖性生长的作用，可能在鼻咽癌发病中具有重要作用。

Effects of RKIP on the proliferation of nasopharyngeal carcinoma cells

ObjectiveTo investigate the effect of Raf kinase inhibitor protein (RKIP) on the proliferation of nasopharyngeal carcinoma (NPC) cells.MethodspcDNA3.1(-)-asRKIP plasmid that constitutively expressed antisense RKIP mRNA and empty plasmid pcDNA3.1(-) were transfected into human NPC cell line 6-10B cells using Lipofectamine2000 to establish cell line with RKIP down-regulation and control cell line, respectively. Methylthiazoletetrazolium (MTT) assay, flow cytometry (FCM) analysis and soft-agar colony formation assay were used to examine the effects of RKIP down-rgulation on proliferation, cell cycle distribution and anchorage independence growth of 6-10B cells, respectively.Results Stably transfected 6-10B cell line with RKIP down-regulation was successfully established. Down-regulation of RKIP in 6-10B cells could promote cell proliferation and anchorage independence growth, and increase the number of G0/G1 phase cells. ConclusionRKIP could inhibit cell proliferation and anchorage independence growth of human NPC cells, suggesting that RKIP might be involved in the pathogenesis of NPC.