| 大片段PCR方法检测CYP2D6~* 5基因分型的选择与优化 |
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| 引用本文： | 柴琳,刘红,许莉,李一石. 大片段PCR方法检测CYP2D6~* 5基因分型的选择与优化[J]. 实用药物与临床, 2017, 0(3): 307-311. DOI: 10.14053/j.cnki.ppcr.201703019 |
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| 作者姓名： | 柴琳 刘红 许莉 李一石 |
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| 作者单位： | 卫生部心血管药物临床研究重点实验室,中国医学科学院阜外医院,北京协和医学院,北京 100037 |
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| 基金项目： | 2010年国家临床重点专科建设项目--《卫生部重点实验室项目》,"重大新药创制"科技重大专项--《心血管创新药物临床研究技术平台建设》(2012ZX09303-008-001) |
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| 摘 要： | 目的经过比较选择更优化的大片段PCR方法,应用到临床研究中对CYP2D6~* 5基因分型检测。方法选择两种大片段PCR方法(传统和巢式大片段PCR)进行比较优化,并对329例健康男性人群进行CYP2D6~* 5基因分型的检测。结果本研究中传统大片段PCR方法分型检测率为92.6%,巢式大片段PCR方法分型检测率为100%;在研究群体中CYP2D6~* 5等位基因的发生频率为3.9%(n=26)。结论优化的大片段PCR方法使CYP2D6~* 5基因分型更高效明确,且巢式大片段PCR方法优于传统大片段PCR方法。
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| 关 键 词： | CYP2D6*5 大片段PCR 巢式大片段PCR 基因分型 |
Selection and optimization of long-PCR method for CYP2D6*5 genotyping assay |
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| Abstract: | Objective To select a long-PCR method which is applied to the clinical research of CYP2D6*5 genotyping.Methods Two long-PCR methods were selected,compared and optimized to detect the genotyping of CYP2D6*5 in 329 Chinese male subjects.Results In our study,successful detection rate of traditional long-PCR method was 92.6%,however the rate of nested long-PCR method was 100% and CYP2D6*5 allele frequency in our study was 3.9% (n=26).Conclusion Optimization made these two long-PCR methods of CP2D6*5 more effective and accurate.Furthermore,the nested long-PCR is working better than the traditional one. |
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| Keywords: | CYP2D6*5 Long-PCR Nested long-PCR Genotyping |
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