MicroRNA在山楂酸诱导A549细胞凋亡中的作用研究

目的探讨MicroRNA在山楂酸诱导肺癌细胞A549凋亡过程中发挥的生物学作用。方法将人的肺癌细胞系A549经过山楂酸干预,并提取处理后A549细胞的总RNA,通过AFFX miRNA表达谱芯片,检测山楂酸作用前后A549细胞中miRNA表达差异情况。通过生物信息学,分析预测miRNA作用的靶蛋白。结果山楂酸作用前后肺癌细胞A549中59个miRNA表达差异显著,其中23个miRNA的表达上调,36个miRNA的表达下调。生物信息学分析预测,由miRNA调控的细胞增殖相关靶蛋白400余个,细胞凋亡相关靶蛋白300余个,其中XIAP是miR-630下游调控的靶蛋白。结论山楂酸可能通过调控miRNA的表达发挥对A549细胞的抑制作用。

Effect of microRNA on apoptosis induced by maslinic acid in lung cancer cell line A549

Objective To investigate the biological effect of microRNA on apoptosis induced by maslinic acid in lung cancer cell line A549.Methods A549 cells were treated with maslinic acid and the total RNA was extracted.Expression pattern of microRNA was examined in A549 cells treated with maslinic acid using AFFX miRNA expression microarray.Target proteins were predicted using bioinformatic methods.Results Totally 59 miRNAs were involved in the inhibition effect of maslinic acid on lung cancer cell,of which 23 miRNAs were significantly up-regulated and 36 miRNAs were down-regulated.Bioinformatic analysis predicted that miRNA regulated more than 400 proliferation-related target proteins and more than 300 apoptosis-related target proteins.XIAP is one of target proteins regulated by miR630.Conclusion Maslinic acid may inhibit A549 cells growth through the regulatory effect of miRNA expression.