白芍总苷对动脉粥样硬化大鼠氧化应激和炎症反应的抑制作用

目的研究白芍总苷(Total glucosides of paeony,TGP)对动脉粥样硬化大鼠氧化应激和炎症反应的抑制作用及其机制。方法应用高脂饲料喂养结合腹腔注射维生素D3建立大鼠动脉粥样硬化(AS)模型,造模过程分别同步灌胃给予TGP 50、100、200 mg/(kg·d)和辛伐他汀(SV)1.8 mg/(kg·d),共12周,并设模型组和正常对照组。采用HE染色法观察主动脉形态结构改变并进行病变分级;比色法测定血清中抗氧化酶活性和丙二醛(MDA)、活性氧簇(ROS)含量;酶联免疫法测定血浆中炎症细胞因子含量。结果与模型组比较,TGP干预组大鼠主动脉病理性形态结构改变呈不同程度改善,其中以TGP 200 mg/(kg·d)组最为显著。TGP 100、200 mg/(kg·d)组病变分级显著降低,血清中抗氧化酶超氧化物歧化酶(SOD)、过氧化氢酶(CAT)]活性显著升高,MDA、ROS含量均降低。其中,TGP 200 mg/(kg·d)组谷胱甘肽过氧化物酶(GSH-Px)活性升高,TGP 100、200 mg/(kg·d)组大鼠血浆中C反应蛋白(CRP)、肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)、IL-6含量降低,且TGP200 mg/(kg·d)组炎症细胞因子(IL-10)含量升高,差异均有统计学意义(P<0.05,P<0.01)。结论 TGP能够通过抑制氧化应激和炎症反应而对动脉粥样硬化表现出一定的保护作用。

Inhibition effects of total glucosides of paeony on oxidative stress and inflammation in atherosclerosis rats

Objective To investigate the inhibition effects of total glucosides of paeony ( TGP) on oxidative stress and inflammation in atherosclerosis ( AS) rats. Methods The rat models of AS were made by feeding high fat diet and injecting vitamin D3 ,and the rats were treated with TGP 50,100 and 200 mg/( kg·d) and simvastatin ( SV) 1. 8 mg/( kg·d) for 12 weeks;meanwhile,the normal control group and model group were set up. The histopathologi-cal change in abdominal aorta was observed and graded by HE staining;the activity of antioxidase and the content of MDA and ROS in serum was determined;the level of inflammatory cytokines in serum was determined. Results Com-pared with model group, the histopathological changes in rats of TGP groups were improved, especially in TGP 200 mg/(kg·d) group. The histopathological degree in TGP groups100,200 mg/(kg·d)] decreased (P<0. 05);the ac-tivity of SOD and CAT in serum increased (P<0. 05,P<0. 01);the content of MDA,NOS and ROS decreased (P<0. 05,P<0. 01). The activity of serum GSH-Px in TGP 200 mg/(kg·d) group increased (P<0. 05). The levels of CRP,TNF-α,IL-1β and IL-6 in plasma of rats in TGP groups 100,200 mg/(kg·d)] decreased significantly (P<0. 05,P<0. 01),while the level of IL-10 in TGP 200 mg/(kg·d) group increased significantly (P<0. 05,P<0. 01). Conclusion TGP has protection effects on rats with atherosclerosis by depressing the oxidative stress and inflammation.