白藜芦醇对间歇低氧大鼠胰岛素抵抗的影响

目的观察白藜芦醇对间歇低氧大鼠胰岛素抵抗的影响,探讨其可能的作用机制。方法 8周龄SD雄性大鼠40只,随机分为5组,对照组(A组)、间歇低氧组(B组)、白藜芦醇低剂量组[C组,6 mg/(kg·d)]、白藜芦醇中剂量组[D组,30 mg/(kg·d)]、白藜芦醇高剂量组[E组,60 mg/(kg·d)]。C组、D组、E组大鼠间歇低氧4周,同时按不同剂量白藜芦醇灌胃。实验结束后,测大鼠空腹胰岛素和血糖水平,取肝脏,采用RT-PCR和Western blot法测定肝脏SIRT1。结果白藜芦醇干预后间歇低氧大鼠的胰岛素抵抗降低,呈剂量依赖性,伴随SIRT1的表达增高,各组间差异有统计学意义(P<0.01)。结论白藜芦醇能减轻间歇低氧大鼠的胰岛素抵抗,呈剂量依赖性,其可能是通过提高SIRT1的表达起作用。

Effects of different doses of resveratrol on insulin resistance in rats with intermittent hypoxia

Objective To investigate the effects of resveratrol on insulin resistance in rats with intermittent hy-poxia and to explore its underlying mechanisms. Methods Forty 8-week-old SD rats were randomly divided into five groups,control group ( group A) ,intermittent hypoxia group ( group B) ,low dose resveratrol group [ group C,6 mg/(kg·d)],median dose resveratrol group [group D,30 mg/(kg·d)],high dose resveratrol group [group E,60 mg/( kg·d) ] . Rats in group C,group D and group E were treated with different doses of resveratrol by gavage and inter-mittent hypoxia for 4 weeks. In the end,the fasting insulin and glucose levels of the rats were measured. The expression of SIRT1 in liver was detected by RT-PCR and Western blot. Results The insulin resistance of rats with chronic inter-mittent hypoxia was decreased,which was dose-dependent,and the expression of SIRT1 was increased,the difference being statistically significant(P<0. 01). Conclusion Resveratrol can reduce the insulin resistance in rats with inter-mittent hypoxia,which is dose-dependent;the mechanism may be by increasing the expression of SIRT1.