路路通酸对乳腺癌MCF-7细胞和宫颈癌C-33A细胞增殖的影响

目的研究路路通酸(BTA)对乳腺癌MCF-7细胞及宫颈癌C-33A细胞增殖的影响。方法采用MTT法检测不同浓度BTA作用不同时间后对MCF-7细胞、C-33A细胞的增殖抑制作用;流式细胞仪检测BTA处理后细胞周期的变化。结果 BTA作用于乳腺癌MCF-7细胞24、48、72 h后的IC50分别为(37.62±1.72)、(27.32±0.99)、(19.19±0.90)μmol/L。BTA作用于宫颈癌C-33A细胞24、48、72 h后的IC50分别为(34.55±0.88)、(27.20±1.03)、(16.74±0.79)μmol/L,BTA对2种细胞增殖有明显抑制作用,且呈浓度时间依赖性(P<0.05),流式结果显示,BTA将MCF-7细胞阻滞在S期,并诱导其凋亡;BTA将C-33A细胞阻滞在G1-S期。结论BTA对乳腺癌MCF-7细胞和宫颈癌C-33A细胞具有较强的增殖抑制作用,其机制与细胞周期阻滞和诱导细胞凋亡有关。

Effect of betulonic acid on proliferation of human breast cancer MCF-7 cells and human cervical cancer C-33A cells

Objective To study the effect of betulonic acid on proliferation of human breast cancer MCF-7 cells and human cervical cancer C-33A cells.Methods MTT assay was used to observe the effect of BTA on growth of cell MCF-7 and cell C-33A in various concentrations for different time periods.Cell cycle and cell apoptosis of these cells were assessed by flow cytometry.Results MTT results showed that the IC50 on MCF-7 cells were (37.62±1.72),(27.32±0.99) and (19.19±0.90) μmol/L after being cultured for 24 h,48 h,and 72 h,while on C-33A cells they were (34.55±0.88),(27.20±1.03) and (16.74±0.79)μmol/L.BTA could significantly inhibit the proliferation of MCF-7 cells and C-33A cells in a dosage-and time-dependent manner (P<0.05).Flow cytometry analysis showed that BTA could induce MCF-7 cells arrest at S phase,and induce apoptosis,while it induced MCF-7 cells arrest at G1-S phase.Conclusion BTA can inhibit the proliferation of MCF-7 cells and C-33A cells;the change of cell cycle and apoptosis may be involved in the process of inhibition.