碱性成纤维细胞生长因子治疗爆震性聋机制的探讨

探讨成纤维细胞生长因子治疗爆震性聋的机制 ,为临床合理用药提供理论依据。爆震豚鼠后 ,一组在耳蜗底回钻孔 ,耳蜗内灌注碱性成纤维细胞生长因子(bFGF) ,另一组动物肌肉注射bFGF ,观察对听神经复合动作电位 (CAP)及听性脑干诱发电位 (ABR)阈值的影响 ;行耳蜗琥珀酸脱氢酶组织化学染色和耳蜗铺片 ,验证bFGF对爆震性聋的疗效。同时观察12 5I标记的bFGF能否透过血迷路屏障。结果发现 ,震后耳蜗内灌注bFGF、肌肉注射bFGF对听阈恢复均有促进作用。但肌肉注射bFGF很难透过血迷路屏障进入内耳 ,推测其作用是间接的 ,可能是通过体内的神经免疫网络对耳蜗起作用。

EXPLORATION OF THE MECHANISM OF THERAPEUTIC EFFECT OF BASIC FIBROBLAST GROWTH FACTOR IN THE TREATMENT OF BLAST-INDUCED DEAFNESS

To explore the mechanism of therapeutic effect of basic fibroblast growth factor (bFGF) in the treatment of blast-induced deafness, and to define its optimal clinical use, bFGF was infused into the guinea pig's cochlea, combined with intramuscular injection of bFGF after being exposed to explosion. The compound action potential (CAP) and auditory brainstem response (ABR) were measured in these animals. 125I labeled basic fibroblast growth factor ( 125I -bFGF) was injected intraperitoneal to the guinea pigs to observe whether it could pass through the blood-labyrinthine barrier. The results showed that bFGF infused to the cochlea might facilitate recovery of hearing loss following acoustic trauma. Basic fibroblast growth factor ( 125I -bFGF) intraperitoneally injected, could not pass through the blood-labyrinthine barrier. However intramuscular bFGF promoted the recovery of hearing, probably indirectly through the neuro-immunity network.