系统性红斑狼疮患者抗双链DNA抗体检测及其与免疫学指标的相关性

目的探讨系统性红斑狼疮患者抗双链DNA（dsDNA）抗体与免疫学指标的相关性。方法收集77例系统性红斑狼疮患者血清,采用短膜虫间接免疫荧光法（CLIFT）及酶联免疫吸附测定（ELISA）检测血清抗dsDNA抗体、总补体、补体C3、补体C4水平。结果 77例系统性红斑狼疮患者50例抗dsDNA抗体阳性,27例抗dsDNA抗体阴性。抗dsDNA抗体阳性组和阴性组患者血清IgG、IgM、IgA水平的差异没有统计学意义（P〉0.05）,抗dsDNA抗体阳性组患者血清总补体、补体C3、补体C4水平明显低于阴性患者（P〈0.05）。抗dsDNA抗体阳性患者血清抗dsDNA抗体水平与总补体、补体C3显著相关（P〈0.05）。结论抗dsDNA抗体可能通过补体旁路途径而非经典途径抑制补体生成。

Detection of anti-double-stranded DNA antibody and its correlation with immunological parameters in patients with systemic lupus erythematosus

Objective To investigate anti-double-stranded DNA（dsDNA）antibodiy and its correlation with immunological pa- rameters in patients with systemic lupus erythematosus.Methods 77 patients with systemic lupus erythematosus were enrolled. Crithidia luciliae immunofluorescence test（CLIFT）and enzyme-linked immunosorbent assay（ELISA）were used to detect serum levels of anti-dsDNA antibodies,total complement,complement C3 and complement C4.Results Among 77 patients with systemic lupus erythematosus,50 were found anti-dsDNA antibody positive,27 were found anti-dsDNA antibody negative.The differences of serum IgG,IgM,IgA levels between anti-dsDNA antibody positive and negative group were not statistically significant（P〈0.05）. The serum levels of total complement,complement C3,complement C4 of patients with positive anti-dsDNA antibody were marked- ly lower than those of patients with negative anti-dsDNA antibody（P〈0.05）.Serum level of anti-dsDNA antibody of patients with positive anti-dsDNA antibody demonstrated significant correlation with serum levels of total complement and complement C3（P〈0.05）.Conclusion The anti-dsDNA antibodies may inhibit complement production via complement alternative pathway rather than classical pathway.