Effects of nerve compression on fast axonal transport in streptozotocin-induced diabetes mellitus |
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Authors: | Dr L B Dahlin K F Meiri W G McLean B Rydevik J Sjöstran |
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Institution: | 1. Laboratory of Experimental Biology Department of Anatomy, University of G?teborg, Box 33031, S-40033, G?teborg, Sweden 2. Department of Pharmacology and Therapeutics, University of Liverpool, Liverpool, UK 4. Department of Orthopaedic Surgery I, University of G?teborg, G?teborg, Sweden 5. Department of Ophthalmology, University of G?teborg, G?teborg, Sweden
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Abstract: | Summary The hypothesis that nerves in diabetes mellitus exhibit an increased susceptibility to compression was experimentally tested.
Inhibition of fast axonal transport was induced by local compression in sciatic nerves of rats with streptozotocin-induced
diabetes mellitus. Fast anterograde axonal transport was measured after application of3H-leucine to the motor neurone cell bodies in the spinal cord. The sciatic nerve as subjected to local, graded compression
in vivo by a small compression chamber. The amount of accumulation of proteins was quantified by calculation of a transport
block ratio. Compression at 30 mm Hg for 3 h induced a significantly greater (p<0.05) accumulation of axonally transported proteins at the site of compression in nerves of diabetic animals (transport block
ratio: 1.01±0.35; n=7) than in nerves of controls (0.67±0.16;n=7). Accumulation was significantly higher in ligature experiments of both control (1.34±0.44;n=8;p< 0.01) and diabetic animals (1.45±0.30;n=8 ;p< 0.05), indicating that the block of transport in compressed nerves was incomplete. Neither sham compressed diabetic (0.50±0.09;n=6) nor control (0.49±0.11;n=6) nerves showed any block of axonal transport. The possible causes of the increased inhibition of fast axonal transport
in diabetic rats are discussed. The results indicate that diabetes may lead to an increased susceptibility of peripheral nerves
to compression. |
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Keywords: | Compression diabetes mellitus streptozotocin axonal transport compression syndromes mononeuropathy neuropathy |
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