平胃散中苍术挥发油的GC/MS分析

复方制剂平胃散出自《太平惠民和剂局方》 ,有燥湿润脾、行气和胃之功效 ,它由 4种单味药即苍术、厚扑、陈皮和甘草组成 ,其挥发油有较强生理活性。其中苍术 (Ｒｈｉｚｏｍａａｔｒａｃｔｙｌｏｄｉｓ)为菊科植物苍术的干燥根茎[1] 。目前 ,对苍术挥发油的化学成分测定一般用ＧＣ或ＧＣ/ＭＳ检测 ,基于气相色谱保留指数或质谱等对部分组分进行定性和定量[2～ 5] 。由于常规ＧＣ或ＧＣ/ＭＳ测定法很难鉴定出色谱峰的纯度 ,有可能导致检测的是几个组分的混合色谱峰 ,在此基础上利用质谱库检索进行定性 ,结果的可靠程度低。本文用ＧＣ/ＭＳ检测…

ANALYSIS OF THE VOLATILE OIL OF RHIZOMA ATRACTYLODIS IN PING-WEI POWDER WITH GC/MS

AIM To detect chemical components of the volatile oil of Rhizoma atractylodis . METHODS Volatile components were analyze by use of GC/MS, heuristic evolving latent projections (HELP) resolution and overall volume integration methods. RESULTS HELP method along with the data from GC/MS can conduct the peak purity examination and resolution of overlapping peaks to obtain pure chromatogram and MS spectrum of each component. Ninty two components were separated and fifty eight of them were qualitatively and quantitatively analyzed, representing about 80 28% of the total content. CONCLUSION The resolved pure chromatogram and MS spectra can greatly enhance the reliability of similar searches in the MS database and thus contribute to the accuracy of the qualitative and quantitative analysis of components.