复合新型骨基质材料体内外成骨能力的组织工程研究

为评价一种新型无机－有机复合骨基质材料（new bone-martrix material,NBM)的体内外成骨能力，取骨髓基质干细胞来源的成骨细胞与材料进行体外复合培养，复合体再行肌内植入，通过相差显微镜，扫描电镜以及组织学观察体内外成骨情况。结果发现，体外培养时成骨细胞与材料可发生良好的粘附、增殖、并分泌大量细胞外基质成分，而细胞－材料复合体肌内植入后4周可见大量淋巴细胞和巨噬细胞浸润，8周仍未见新生骨组织生成。作者认为NBM的这种体内外成骨能力的差异可能与NBM具有免疫原性，引起宿主免疫反应，从而丧失体内成骨环境有关。

TISSUE ENGINEERING STUDIES ON BONE FORMATION USING NEW EXTRACELLULAR MATRIX MATERIAL

To design and manufacture a new bone matrix material (NBM) composed of both organic and inorganic materials for bone tissue engineering, the osteogenic potential of NBM combined with osteoblasts was studied in vitro and in vivo. Osteoblasts from bone marrow stromal cells of New Zealand rabbit were cultured with NBM in vitro , then the materials combined with osteoblasts were implanted into the skeletal muscles of rabbits. The osteogenic potential of NBM was evaluated using contrast microscope, scan electromicroscope and histological examination. Osteoblasts could attach and proliferate well in the NBM, secrecting lots of extracellular matrix, while in vivo experiment of the NBM in osteoblasts group showed that a large number of lymphacytes and phagocytes invaded into the inner of the material in the rabbit skeletal muscle beds after 4 weeks of implantation, no new bone formation was observed after 8 weeks. The different osteogenic potential expressed by NBM between in vitro and in vivo may be due to the immunogenity of NBM which causes cellular immunoreaction to destroy the osteogenic environment. More attention should be paid to the immunoreaction problem in tissue engineering between the host and organic inorganic composite materials.