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宁夏大蓟提取物不同极性部位对5种革兰阴性菌的体外抑菌活性研究
引用本文:叶莉,杨凤琴,张志宁,杨建宏,高兰月,高亦珑. 宁夏大蓟提取物不同极性部位对5种革兰阴性菌的体外抑菌活性研究[J]. 辽宁中医杂志, 2011, 0(10): 2053-2054
作者姓名:叶莉  杨凤琴  张志宁  杨建宏  高兰月  高亦珑
作者单位:宁夏医科大学高职学院;宁夏医科大学检验学院;宁夏医科大学药学院;
基金项目:宁夏医科大学面上课题(XM2010046)
摘    要:目的:观察宁夏大蓟提取物的不同极性部位对5种革兰阴性菌体外抑菌活性作用。方法:大蓟水提取物、大蓟正丁醇提取物、大蓟乙酸乙酯提取物用试管二倍稀释法联合琼脂平板法,进行体外抑菌试验研究。结果:大蓟水提取物、大蓟乙酸乙酯提取物对5种革兰阴性菌最小抑菌浓度(MIC)均为﹥1.00 mg.mL-1;大蓟正丁醇提取物对5种革兰阴性菌有抑菌作用,对大肠杆菌、铜绿假单胞杆菌MIC均为1.0mg.mL-1;对伤寒沙门菌、变形杆菌、福氏痢疾杆菌MIC均为0.5mg.mL-1。结论:大蓟正丁醇提取物对5种革兰阴性菌有抑菌作用,大蓟水提取物、大蓟乙酸乙酯提取物无抑菌作用。

关 键 词:宁夏大蓟提取物的不同极性部位  G-菌体外抑菌试验  MIC

Study on the Antimicrobial Activity of Different Polar Fractions From Extracts of Ningxia Cirsium Japonicum on the Five Gram Negtive Bacteria in Vitro
YE Li,YANG Feng-qin,ZHANG Zhi-ning,YANG Jian-hong,GAO Lan-yue,GAO Yi-long. Study on the Antimicrobial Activity of Different Polar Fractions From Extracts of Ningxia Cirsium Japonicum on the Five Gram Negtive Bacteria in Vitro[J]. Liaoning Journal of Traditional Chinese Medicine, 2011, 0(10): 2053-2054
Authors:YE Li  YANG Feng-qin  ZHANG Zhi-ning  YANG Jian-hong  GAO Lan-yue  GAO Yi-long
Affiliation:YE Li1,YANG Feng-qin2,ZHANG Zhi-ning1,YANG Jian-hong3,GAO Lan-yue1,GAO Yi-long3(1.Higher Vocational College of NingXia Medical University,Yinchuan 750004,Ningxia,China,2.Institute of Laboratory Medicine of NingXia Medical University Yinchuan 750004,3.Pharmacy College of NingXia Medical University,China)
Abstract:Objective:To observe the antimicrobial activity of different polar fractions from extracts of Ningxia Cirsium japonicum on five gram negtive bacteria in vitro.Methods:The antimicrobial test of water-extract of Cirsium japonicum,n-butyl alcohol-extract of Cirsium japonicum and ethyl acetate-extract of Cirsium japonicum was performed by the tube double dilution method combined with agar plate method in vitro.Results:The minimum inhibitory concentration(MIC) of water-extract of Cirsium japonicum and ethyl acet...
Keywords:different polar fractions from extracts of Ningxia Cirsium japonicum  G-antimicrobial test in vitro  MIC  
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