| HPLC法测定南丹参中丹酚酸B的含量 |
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| 引用本文： | 刘丹,余良忠,文萍.HPLC法测定南丹参中丹酚酸B的含量[J].辽宁中医杂志,2011(9):1872-1873. |
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| 作者姓名： | 刘丹 余良忠 文萍 |
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| 作者单位： | 江西省中医药研究院; |
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| 基金项目： | 江西省卫生厅中医药科研课题(2008A092) |
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| 摘 要： | 目的:建立南丹参中丹酚酸B的含量测定方法。方法:色谱柱:Dikma Diamonsil C18柱(4.6mm×250mm,5μm),流动相:甲醇-乙腈-1.5%甲酸溶液(16∶16∶68),流速:1mL/min,检测波长286nm,柱温:室温。结果:线性范围为11.984～299.6 mg.mL-1(r=0.9998),平均回收率为99.57%,RSD=1.83%,精密度RSD=1.66%(n=6),重复性RSD=1.08%(n=6),稳定性RSD=1.60%。结论:方法稳定、可靠,可作为该药材的质量控制方法。
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| 关 键 词： | 南丹参 丹酚酸B 高效液相色谱 |
Determination of Salvianolic Acid B in Radix Salvie Bowleyanae by HPLC |
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| LIU Dan,YU Liang-zhong,WEN Ping.Determination of Salvianolic Acid B in Radix Salvie Bowleyanae by HPLC[J].Liaoning Journal of Traditional Chinese Medicine,2011(9):1872-1873. |
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| Authors: | LIU Dan YU Liang-zhong WEN Ping |
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| Institution: | LIU Dan,YU Liang-zhong,WEN Ping(Jiangxi Institute of Traditional Chinese Medicine,Nanchang 330046,Jiangxi,China) |
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| Abstract: | Objective:To establish the method for determination content of salvianolic acid B in Radix salvie bowleyanae.Methods:The chromatographic conditions were as follows: a Dikma Diamonsil C18(4.0mm×250mm,5μm) column,a mobile phase of methanol-acetonitrile-1.5%formic acid(16:16:68).and a UV detector at 286nm.Results:A linear ranger of 11.984～299.6mg·mL-1(r=0.9998),the average recovery of 99.57%(RSD=1.83%),precision with RSD of 1.66%(n=6),repetition with RSD of 1.08%(n=6)and stability with RSD of 1.60% were obtained.Conclusion:The method is accurate,reliable,and can be used for quality control of the production. |
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| Keywords: | radix salvie bowleyanae salvianolic acid B HPLC |
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