黑木耳粗提物在大鼠急性肾功能衰竭过程中的作用

 目的： 探讨黑木耳粗提物对大鼠腹腔注射顺铂诱发急性肾功能衰竭的作用及其可能机制。方法：将健康雄性Wistar大鼠32只随机分为对照组、模型组、黑木耳组及尿毒清组，每组8只。采用RT-PCR方法检测各组大鼠肾脏组织内皮素1（ET-1） mRNA的表达水平。同时测定血尿素氮（BUN）、肌酐（Cr）的含量及血气指标。结果：模型组与尿毒清组中ET-1 mRNA的表达，BUN和Cr的含量均高于对照组和黑木耳组（P<0.05），而黑木耳组中ET-1 mRNA的表达与对照组比较差异无统计学意义（P>0.05）。血气分析也表明，黑木耳组发生了代偿性的酸碱平衡紊乱，且血K+浓度与对照组比较差异无统计学意义（P>0.05）。结论：黑木耳粗提物在急性肾功能衰竭过程中可能通过影响血管活性物质的合成与释放，调节体内电解质和酸碱的平衡而发挥保护作用。

Effect of black fungus extract on acute renal failure in rats

AIM：To investigate the effect of the extract of black fungus on acute renal failure (ARF) induced by injection of cisplatin  in rats. METHODS：Thirty-two healthy male Wistar rats were randomly divided into 4 groups (n=8)：control group, model group, black fungus group and niaoduqing group. The mRNA expression of endothelin-1 (ET-1) was detected by RT-PCR. Blood urea nitrogen (BUN) and creatinine (Cr) in serum were measured. The blood gas was also analyzed. RESULTS：Compared with model group and niaoduqing group, the mRNA expression of ET-1, BUN and serum Cr in control group and black fungus group increased obviously (P<0.05). No significant difference between control group and black fungus group was observed (P>0.05). The results of blood gas analysis indicated that there were compensatory disorders of acid-base balance in black fungus group. CONCLUSION：The extract of black fungus may improve the renal function by influencing the production and release of vasoactive substance, and the extract also regulates the balance of electrolyte and acid-base balance.