癌基因c-erbB2、c-myc和抑癌基因p16、p53在口腔鳞癌中的蛋白表达及其协同作用

目的：探讨癌基因c—erbB2、c-myc和抑癌基因p16、p53在口腔鳞癌(OSOC)中的蛋白表达及其协同作用。方法：用免疫组化结合图像分析对口腔鳞癌中4种基因的蛋白表达进行定性、定位、定量研究。结果：口腔鳞癌中c—erbB2、c—myc、p16和p53的蛋白表达阳性率依次为46．67％、60％、86．67％和63。33％。肿瘤部位不同，erbB2蛋白表达有显著性差异；腭癌和口底癌中的erbB2蛋白表达都明显高于唇癌和牙龈癌中的erbB2表达。c-myc蛋白表达与p16蛋白表达之间具有显著性相关。结论：以上4种基因的蛋白表达增高在口腔鳞癌发生发展中具有重要作用，c—erbB2蛋白过表达在腭癌和牙龈癌中具有更重要的意义；c—myc和p16蛋白表达间具有一定的内在联系。

Protein expression and cooperation of oncogene c-erbB2,c-myc and anti-oncogene p16,p53 in oral squamous cell carcinomas

Objective:To study the expression and cooperation of oncogene (c-myc and c-erbB2) and anti-oncogene (p16 and p53) in oral squamous cell carcinomas(OSCC). Methods: Using immunohistochemical technique and image analysis, the expression of protein of c-erbB2,c-myc, p16 and p53 were studied in 30 OSCC patients and 5 normal oral mucosa tissues about the nature, location and quantity. Results: The protein expression of c-erbB2,c-myc,p16 and p53 were 46.67%,60%,86.67% and 63.33%,respectively. Protein expression of c-erbB2 had significant difference in different tumor positions (P<0.05),The protein expression of erbB2 in carcinoma of palate and floor of mouth were obviously higher than the those in lip cancer and gingival carcinoma (P<0.05).There was significant correlation between p16 and c-myc protein expression (P<0.01). Conclusion: The protein overexpression of c-erbB2,c-myc,p16 and p53 may play important roles in the tumorigenesis and development of OSCC, and the overexpression of c-erbB2 protein has more important significance in tumorigenesis of carcinomas of palate and floor of mouth. There is certain internal relation between p16 and c-myc protein expression.