哮喘患者IL-13基因多态性与IL-13、TIgE水平相关性研究

目的:探讨白细胞介素13(IL-13)基因内含子区+1923C/T多态性与哮喘患者外周血单个核细胞(PBMC)产IL-13、血浆总IgE(TIgE)水平及其相关性。方法:用聚合酶链反应和限制性片段长度多态性(PCR/RFLP)方法检测哮喘组与对照组+1923C/T位点多态性。IL-13、血浆总IgE采用ELISA法。结果:+1923位点等位基因C、T频率在两组间分布的差异具有显著性(X2=9.30,P<0.01);等位基因T与哮喘关联,OR(T/C)=1.87,95%CI=1.25-2.80,P<0.01。两组基因型(TT、CT、CC)频率的分布差异亦有显著意义(X2=9.92,P<0.01)。其优势比:OR(TT/CC)=3.76,95%CI=1.52-9.29,P<0.01;OR(CT/CC)=2.10,95%CI=1.11-3.95,P<0.05;OR(TT/CT)=1.79,95%CI=0.77-4.19,P>0.05。哮喘组中TT、TC基因型人群PBMC产IL-13及TIgE水平与同组及对照组CC基因相比较差异均有显著性(P<0.01)。结论:IL-13基因+1923位点多态性是影响哮喘的重要候选基因,T等位基因与哮喘关联。

Association between IL-13 gene polymorphism and level of IL-13 and TIgE in patients with asthma

Objective:To investigate the correlation between+1923(C/T) locus polymorphism of IL-13 gene intron and the IL-13 levels from cultured peripheral blood mononuclear cell supernatant, the levels of plasma TIgE of patients with asthma. Methods:Polymerase chain reaction/restriction fragment length polymorphism(PCR/RFLP) technique were used to determine + 1923 locus polymorphism in asthma and control group.IL-13 and IgE were determined by ELISA.Results:The frequency of allele C,T of + 1923 locus had significant difference between two groups (X2 = 9.30, P<0.01) .allele T was associated with asthma,OR(T/C) = 1.87,95%CI = 1.25-2.80, P<0.01 .The genetype(CC,CTandTT) distribution was significantly different between two groups (X2= 9.92,P<0.01),OR(TT/CC) =3.76,95%CI= 1.52 - 9.29,P < 0.01;OR(CT/CC) =2.10,95%CI= 1.11-3.95,P < 0.05;OR(TT/CT)=1.79,95%CI=0.77-4.19,P>0.05. The levels of IL-13 and TIgE of TT\TC geneotypes were significantly higher than those of CC genotype respectively(P<0.01). Conclusion:The + 1923 locus polymorphism of IL-13 gene intron may be an important candidate gene for asthma. Allele T is associated with asthma.