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Spread of Stenotrophomonas maltophilia colonization in a pediatric intensive care unit detected by monitoring tracheal bacterial carriage and molecular typing |
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| Authors: | P. Lanotte S. Cantagrel L. Mereghetti S. Marchand N. Van der Mee J. M. Besnier J. Laugier R. Quentin |
| Affiliation: | Département de Microbiologie Médicale et Moléculaire, EA 3250, Facultéde Médecine de Tours and Laboratoire de Bactériologie, Hôpital Bretonneau et Clocheville, CHU de Tours, 2, boulevard Tonnellé, F-37044 Tours,;UnitéPédiatrique de Soins Intensifs, Hôpital Clocheville, CHU de Tours, Tours and;Comitéde Lutte contre les Infections Nosocomiales, CHU de Tours, Tours, France;Tel: + 33 2 47 47 80 56;Fax: + 33 2 47 47 36 10E-mail: |
| Abstract: | In our pediatric intensive care unit in Tours (France), intubated and ventilated inpatients are systematically monitored for tracheal bacterial colonization twice a week. This led us to detect five patients colonized with Stenotrophomonas maltophilia over a 4-month period. Molecular typing of the isolates using random amplified polymorphism DNA (RAPD) and pulsed-field gel electrophoresis (PFGE) confirmed that four of the five isolates were genetically related. The strict isolation of carriers and improvements in hygiene measures stopped the spread. This systematic strategy prevented pulmonary nosocomial infections or allowed their early detection. Moreover, it has made it possible to assess the efficiency of care practices continuously. |
| Keywords: | Stenotrophomonas maltophilia nosocomial infections monitoring bacterials carriage RAPD PFGE |