羧甲基壳聚糖钙对染铅小鼠排铅作用的初步研究

目的观察羧甲基壳聚糖钙对染铅小鼠体内铅、钙、锌含量及其抗氧化能力的影响。方法将一级昆明种小鼠分为正常对照组、碳酸钙对照组、染铅模型组、羧甲基壳聚糖钙低、中、高剂量实验组及其依地酸钙钠对照组,饮用醋酸铅水溶液建立小鼠预防性铅中毒模型,同时灌胃给予受试物。连续30d后,采血,取肝脏、脑、股骨,用原子吸收分光光度计测定其铅、锌、钙含量。同时,用试剂盒测定小鼠肝组织中的丙二醛（MDA）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、总抗氧化能力（T-AOC）值。结果羧甲基壳聚糖钙能使染铅小鼠体内血液、全脑、肝脏、股骨中铅含量由约1．56μg／g、13．38μg／g、16．15μg／g、1011．62μg／g显著降低至0．50μg／g、5．57μg／g、5．64μg／g、457．86μg／g,并使股骨钙含量显著升高,对小鼠全血和其他组织内锌含量无影响;羧甲基壳聚糖钙能使染铅小鼠肝脏中MDA值显著降低,SOD、GSH-Px、T-AOC活性显著升高。结论羧甲基壳聚糖钙能显著促进染铅小鼠排铅,提高骨钙含量,增强抗氧化能力。

Lead expelling effect of carboxymethyl chitosan calcium in lead poisoned mice

OBJECTIVE: To observe the effects of carboxymethyl chitosan calcium (CCC) on concentration of lead, calcium and zincum, and the liver antioxidative capacity in lead poisoned mice. METHODS: Mice were randomly divided into 7 groups, including normal group, calcium carbonate group, lead-model group, and three experimental groups treated with CCC in three different doses, and the CaNa2EDTA positive control group. The lead poisoned mice model was established by giving water contained with lead acetate. CCC was administrated to mice i.g. once a day. Thirty days later, mice were killed and the concentrations of lead, calcium and zincum in blood, liver, brain and femur were determined by atomic absorption spectrophotometer. Maleicdialdehyde (MDA), total antioxidative capacity (T-AOC), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) activities in liver were measured by using assay kit. RESULTS: CCC significantly reduced the concentration of lead in blood, brain, liver and femur from about 1.56 microg/g, 13.38 microg/g, 16.15 microg/g, 1011.62 microg/g to about 0.50 microg/g, 5.57microg/g, 5.64 microg/g, 457.86 microg/g, and markedly increased the concentration of calcium in femur in lead poisoned mice. CCC had no significant side-effects on concentration of zincum in lead poisoned mice. The antioxidative profile was favorably changed as manifested by decreasing the level of MDA, increasing the activities of SOD, GSH-Px and T-AOC in livers of the in lead poisoned mice. CONCLUSION: CCC might significantly advance the excretion of lead, increase the concentration of calcium in femur and the antioxidative capacity in lead-loaded mice.