| 氧化低密度脂蛋白对人血管平滑肌细胞生长状况的影响 |
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| 引用本文： | 赵高峰. 氧化低密度脂蛋白对人血管平滑肌细胞生长状况的影响[J]. 郑州大学学报(医学版), 2002, 37(4): 436-438 |
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| 作者姓名： | 赵高峰 |
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| 作者单位： | 郑州大学第一附属医院心胸外科,郑州,450052 |
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| 基金项目： | 国家科学技术部攀登计划支持项目 (1997.1～ 2 0 0 0.12 ) |
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| 摘 要： | 目的：探讨氧化低密度脂蛋白（oxidized low density lipoprotein ox-LDL）对培养的人血管平滑肌细胞（smooth muscle cell,SMC）生长状况的影响。方法：利用密度梯度速离心分离纯化人血浆LDL，体外氧化生成ox-LDL,不同浓度的ox-LDL作用于培养的人SMC不同时间，从不同的角度观察其对SMCT生长状况的影响。结果：ox-LDL对SMC的作用具有时间浓度依赖性，低浓度的ox-LDL促SMC增生及表型转换，高浓度时则诱导SMC发生凋亡，甚至死亡。结论：ox-LDL可能在人动脉粥榇硬化的发生发展过程中起重要作用。
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| 关 键 词： | 氧化低密度脂蛋白 平滑肌细胞 生长发育 动脉粥样硬化 |
| 修稿时间： | 2001-07-20 |
Influence of ox-LDL on the cultured human vascular SMC |
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| ZHAO Gaofeng. Influence of ox-LDL on the cultured human vascular SMC[J]. Journal of Zhengzhou University: Med Sci, 2002, 37(4): 436-438 |
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| Authors: | ZHAO Gaofeng |
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| Affiliation: | ZHAO Gaofeng Department of Cardiovascular Thoracic Surgery,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052 |
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| Abstract: | Aim: To study the influence of ox-LDL (oxidized low density lipoprotein) on the cultured SMC(smooth muscle cell). Methods: Through NaBr density gradient centrifugation, LDL was isolated and purified from human plasm. ox-LDL was produced from LDL by being incubated with CuSO 4.ox-LDL was then added to the SMC medium at different concentration and incubated for different time. The influence of ox-LDL on SMC was investigated from several aspects. Results: ox-LDL could promote hyperplasia and transformation of SMC at lower concentration and induce SMC apoptosis and even death at higher concentration. Conclusion: ox-LDL plays an important role in the pathogenesis and developement of atherosclerosis. |
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| Keywords: | oxidized low density lipoprotein smooth muscle cell growth and development |
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