The pursuit of resin-dentin bond durability: Simultaneous enhancement of collagen structure and polymer network formation in hybrid layers |
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Authors: | Thiago Henrique Scarabello Stape Mustafa Murat Mutluay Leo Tjäderhane Emilia Uurasjärvi Arto Koistinen Arzu Tezvergil-Mutluay |
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Institution: | 1. Department of Restorative Dentistry and Cariology, Adhesive Dentistry Research Group, Institute of Dentistry, University of Turku, Turku, Finland;2. Turku University Hospital, TYKS, University of Turku, Turku, Finland;3. Department of Prosthodontics and Clinical Dentistry, Institute of Dentistry, University of Eastern Finland, Kuopio, Finland;4. Department of Oral and Maxillofacial Diseases, University of Helsinki, Helsinki, Finland and Helsinki University Hospital, Helsinki, Finland;5. Research Unit of Oral Health Sciences and Medical Research Center Oulu (MRC Oulu), Oulu University Hospital and University of Oulu, Oulu, Finland;6. University of Eastern Finland, SIB Labs, Kuopio, Finland |
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Abstract: | ObjectiveImperfect polymer formation as well as collagen’s susceptibility to enzymatic degradation increase the vulnerability of hybrid layers over time. This study investigated the effect of new dimethyl sulfoxide (DMSO)-containing pretreatments on long-term bond strength, hybrid layer quality, monomer conversion and collagen structure.MethodsH3PO4-etched mid-coronal dentin surfaces from extracted human molars (n = 8) were randomly treated with aqueous and ethanolic DMSO solutions or following the ethanol-wet bonding technique. Dentin bonding was performed with a three-step etch-and-rinse adhesive. Resin-dentin beams (0.8 mm2) were stored in artificial saliva at 37 °C for 24 h and 2.5 years, submitted to microtensile bond strength testing at 0.5 mm/min and semi-quantitative SEM nanoleakage analysis (n = 8). Micro-Raman spectroscopy was used to determine the degree of conversion at different depths in the hybrid layer (n = 6). Changes in the apparent modulus of elasticity of demineralized collagen beams measuring 0.5 × 1.7 × 7 mm (n = 10) and loss of dry mass (n = 10) after 30 days were calculated via three-point bending and precision weighing, respectively.ResultsDMSO-containing pretreatments produced higher bond strengths, which did not change significantly over time presenting lower incidence of water-filled zones. Higher uniformity in monomer conversion across the hybrid layer occurred for all pretreatments. DMSO-induced collagen stiffening was reversible in water, but with lower peptide solubilization.SignificanceImproved polymer formation and higher stability of the collagen-structure can be attributed to DMSO’s unique ability to simultaneously modify both biological and resin components within the hybrid layer. Pretreatments composed of DMSO/ethanol may be a viable-effective alternative to extend the longevity of resin-dentin bonds. |
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Keywords: | Dimethyl sulfoxide DMSO Ethanol Adhesive systems Bond strength Microtensile Wet bonding Etch-and-rinse Micro Raman Degree of conversion |
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