急性弓形虫感染对雄性小鼠睾丸生精功能影响的初步研究

目的:探讨弓形虫急性感染对雄性小鼠睾丸生精功能的影响。方法:将26只成年雄性小鼠随机均分为感染组和正常对照组;感染组腹腔注射0.3ml1×103/ml弓形虫速殖子,感染小鼠睾丸,正常组腹腔注射等量生理盐水,两组睾丸制成直接印片及病理切片,观察小鼠生精细胞的病理变化及弓形虫侵入生精细胞情况。同时比较两组睾丸乳酸脱氢酶同工酶、精子密度、精子活动率、精子畸形率。结果:感染组睾丸乳酸脱氢酶同工酶、精子密度、精子活动率、精子畸形率分别为53.19±18.04、(15.01±2.42)×106/ml、(8.26±2.57)%、(17.69±11.91)%,正常组为68.71±17.79、(23.87±6.66)×106/ml、(13.21±2.82)%、(11.30±6.60)%,两组比较差异均有显著性(P<0.05)。结论:弓形虫感染对雄性小鼠睾丸生精功能有一定的影响。

Acute Toxoplasma Gondii Infection Affects the Reproductive Function of Male Mice

OBJECTIVE: To investigate the influence of acute Toxoplasma gondii infection on the reproductive function of male mice. METHODS: Twenty-six adult male mice were randomized into an infection and a control group of equal number. Acute Toxoplasma gondii infection was induced in the testes of the former, while abdominal injection of normal saline given to the latter. Cell imprints and pathological sections were obtained to observe the pathological changes and Toxoplasma gondii invasion in the spermatogenic cells and to compare the testicular LDH-X, sperm concentration and motility and the number of deformed spermatozoa between the two groups. RESULTS: The testicular LDH-X, sperm concentration and motility and the number of deformed spermatozoa were 53.19 +/- 18.04, (15.01 +/- 2.42) x 10(6)/ ml, (8.26 +/- 2.57) % and (17.69 +/- 11.91) % in the infection group, as compared with 68.71 +/- 17.79, (23.87 +/- 6.66) x 10(6)/ ml, (13.21 +/- 2.82) % and (11.30 +/- 6.60) % in the control, with significant differences between the two groups (P < 0 05). CONCLUSION: Acute Toxoplasma gondii infection affects the reproductive function of male mice.