邻苯二甲酸二丁酯对F1 代仔鼠睾丸损害的可逆性恢复特性

目的研究和探讨邻苯二甲酸二丁酯(DBP)对F1代仔鼠睾丸损害是否具有可逆性恢复的特性。方法在宫内暴露和哺乳期(妊娠1d至仔鼠出生21d),每日每kg体重0、50、250和500mg DBP灌胃染毒孕鼠(每组10只),从解剖学、病理学等角度观察DBP对不同发育阶段(出生14、21和70d)F1代雄性大鼠的睾丸损害。结果与对照组相比,虽然不同发育阶段大鼠睾丸重量及系数没有明显统计学意义,但与对照组相比较,250、500mg／L组出生14d大鼠生精上皮变薄,Sertoli细胞数量明显减少(分别减少至对照组的84％和86％),并出现空泡样改变;出生21d时,Sertoli细胞数量和形态基本恢复正常,但有少量生精细胞脱落;至出生70d时,个别大鼠睾丸出现曲细精管变性、生精上皮萎缩等不可逆性损害。结论DBP宫内暴露和哺乳期染毒可损害F1代大鼠睾丸Sertili细胞,但随着仔鼠的发育,DBP致Sertoli细胞损害作用具有部分可逆性恢复的特性。

Reversibility of adverse effects of di-n-butyl phthalate on F1 generation rat testes

Objective To study the reversibility of di n butyl phthalate (DBP) effects on F 1 generation rat testes Methods Pregnant rats were treated with different dose of DBP (0, 50, 250 and 500 mg per kg per day) by gavage from GD1 to PND21 The adverse effects of DBP on testes of F 1 male rats in different developmental period (PND14, 21 and 70) were observed by anatomy and pathological methods Results There was no difference in rat testis weight and testis/body weight between DBP treated group and the control From the results of pathology and sertoli cell counting, comparing with the control, thinner seminiferous epithelium, decreased cell number and vacuole cells were observed in PND14 male DBP treated rats In PND21 rats, the number and form of sertoli cells were recovered and few exfoliated spermatogenic cells were found When maturating to PND70, few rats were found irreversibilitable damages such as seminiferous tubule degeneration, seminiferous epithelium atrophy, etc Conclusion These results suggest that adverse effects of DBP on young rat testes should be reversibility