基质金属蛋白酶组织抑制因子-1,-2在脑膜瘤中的表达及意义

目的研究基质金属蛋白酶组织抑制因子-1,-2(tissue inhibitor ofMMP,TIMP-1,-2)与脑膜瘤的关系.方法采用免疫组化S-P法(Streptavidin-Peroxidase)检测10例正常蛛网膜组织、28例良性脑膜瘤和32例恶性脑膜瘤组织中TIMP-1及TIMP-2的表达水平.结果 TIMP-1在正常蛛网膜组织、良性脑膜瘤和恶性脑膜瘤中的阳性表达率分别为100%(10/10)、89.3%(25/28)和12.5%(4/32),其表达强度经检验显示呈逐次降低趋势,各组间的差异有统计学意义(P＜0.05).TIMP-2在正常蛛网膜组织、良性脑膜瘤和恶性脑膜瘤中的阳性表达率分别为90.0%(9/10)、92.9%(26/28)和18.8%(6/32),其表达强度经检验显示呈逐次降低趋势,其中恶性脑膜瘤与良性脑膜瘤之间的差异有统计学意义(P＜0.01).结论 TIMP-1,TIMP-2在恶性脑膜瘤中的表达强度低于良性脑膜瘤和正常蛛网膜组织,表明TIMP-1和TIMP-2表达水平的下调在脑膜瘤的侵袭、恶变过程中起一定作用.

Expression and significance of TIMP-1, TIMP-2 in meningiomas

[Objective] To study the relationship between TIMP-1,TIMP-2(tissue inhibitor of MMP,TIMP -1,-2) and human meningiomas. [Method] Streptavidin-Peroxidase (S-P) immunostaining technique was used to examine the expression of TIMP-1 and TIMP-2 in all of the tissues. [Results] In normal arachnoid tissues, benign and malignant meningiomas, the positive expression of TIMP-1 were 100%(10/10), 89.3%(25/28) and 12.5%(4/32) respectively. There was significant difference between normal arachnoid tissues and benign meningiomas (P <0.05). There was also significant difference between malignant meningiomas and benign meningiomas (P <0.01). Positive expression rates of TIMP-2 in normal arachnoid tissues, benign meningiomas and malignant meningiomas were 90%(9/10), 92.9%(26/28) and 18.8%(6/32) respevtively. The expression level of TIMP-2 in malignant meningiomas was lower than that in benign meningiomas and normal arachnoid tissues (P <0.01). [Conclusions] The decreased expression of TIMP-1 and TIMP-2 in meningiomas might relate to the development, malignance and invasion of meningiomas