猪干扰素-γ基因表达产物的纯化与复性研究

目的：纯化、复性猪干扰素-γ基因（poIFN-γ）表达产物并分析其抗病毒活性。方法：用异丙基-β—D-硫代半乳糖苷（Isopropylthio—β—D—galactoside，IPTG）诱导原核表达载体pQE30／poIFN-γ，超声裂解包涵体，尿素溶解、Ni—NTA纯化和透析复性后，对所得的蛋白进行抗病毒活性检测。结果：经复性纯化的目的蛋白纯度达95％以上，在PK15细胞上抗滤泡性口炎病毒比活性为6．2×10^5u／mg。结论：获得PoIFN-γ活性蛋白，为进一步研究应用奠定基础。

PURIFICATION AND RENATURATION OF THE EXPRESSION PRODUCT OF PORCINE INTERFERON GAMMA GENE

Objective： To purify and renaturate poIFN-γ expressed product and to study its antiviral activity. Methods： After pQE30/poIFN-γ was induced with isopropye-β-D-thiogalactoside （IPTG）, the inclusion bodies were isolated with sonicate, purified by using Ni-NTA agarose and renaturated with dialysis, then its antiviral activity was tested. Results： The purified protein reached about 95%. Its specific antiviral activity was about 6.2×10^5u/mg on PK15/VSV test system. Conclusion： The active poIFN-γ protein has been obtained,which is a good fowndation for future research and application.